Polyhydroxyalkanoates (PHA) Strain Engineering Service

Polyhydroxyalkanoates (PHA) is a highly promising biodegradable material , and the key to its industrialization lies in the breakthrough enhancement of strain performance. CD Biosynsis is deeply rooted in the field of synthetic biology , specializing in the precision engineering and optimization of PHA strains. We leverage core gene editing technologies to offer professional services ranging from metabolic pathway elucidation to experimental validation.

We analyze the carbon metabolic network of high-yield strains such as Cupriavidus necator , and utilize techniques like CRISPR-Cas9 precision editing and Calvin Cycle activation. Our goal is to enhance PHA's carbon source conversion rate, yield, and purity. Backed by a comprehensive laboratory research platform, we provide clients with scientifically rigorous strain engineering solutions and experimental data support , helping to drive the iterative upgrade of PHA bioproduction technology and establish core strain foundations for the biomaterials industry.

Pain Points

In current PHA production, inadequate strain performance is the critical bottleneck restricting efficiency. This is mainly manifested in:

• Low Carbon Source Conversion Efficiency: Conventional strains typically achieve only a 57% carbon source conversion rate during fermentation, resulting in substantial unused carbon source and directly elevating raw material costs.
• Unstable Product Purity: Impurities such as proteins and metabolic byproducts can easily be mixed into the fermentation product , causing purity fluctuations that compromise the material's mechanical properties and restrict its use in high-end applications.
• Metabolic Pathway Redundancy: The strain's natural metabolic network contains non-essential branches , leading to dispersed energy and carbon flux allocation, which limits PHA synthesis efficiency.
• Narrow Carbon Source Adaptability: Most strains poorly utilize cheap alternative carbon sources (such as oils, lignocellulosic hydrolysates) , making it difficult to reduce raw material dependency.

The core of these issues lies in the strain's inherent metabolic characteristics , necessitating performance breakthroughs through precision engineering.

Solutions

CD Biosynsis addresses the core pain points of PHA strains by constructing multi-dimensional engineering strategies , with systematic experimental validation to optimize effectiveness:

Cupriavidus necator Oil Utilization Enhancement

Gene editing is employed to strengthen the strain's transport and degradation capabilities for oil-based carbon sources. We test the conversion efficiency of substrates like palm oil and waste cooking oil in experimental systems , broadening the range and economic viability of carbon source utilization.

Calvin Cycle Pathway Optimization

High-efficiency CO2 fixation-related enzyme genes are introduced to reconstruct the carbon metabolic pathway. We validate the potential for secondary utilization of byproduct CO2 under controlled experimental conditions, thereby boosting the overall carbon source conversion rate.

CRISPR-Cas9 Metabolic Pathway Editing

Key genes restricting PHA synthesis are precisely targeted and edited , redundant metabolic branches are knocked out , and byproduct formation is reduced. The impact on yield and purity enhancement is verified in experimental models.

Strain Performance Evaluation System

A multi-index evaluation method is established, encompassing growth rate, carbon source consumption, product accumulation, and purity analysis , to comprehensively analyze the engineered strain's overall performance.

The solution is grounded in experimental data , focuses on the strain's intrinsic performance optimization , and provides a referenceable technical roadmap and result analysis.

Advantages

Choosing CD Biosynsis's PHA strain engineering service offers the following core value:

High Technical Specificity

We focus on the precise engineering of strain metabolic characteristics , designing solutions specifically for critical steps like carbon source conversion and product synthesis , avoiding generalized technology application.

Rigorous Experimental Data

Utilizing a standardized laboratory platform, data is acquired through three or more replicate experiments , including a full set of metrics such as strain growth curves, product concentration, and purity analysis , ensuring the reference value of the results.

Leading Editing Efficiency

We employ an optimized CRISPR-Cas9 system , adjusting the editing strategy to suit the genetic characteristics of high-yield PHA strains. This boosts the efficiency of positive clone screening and shortens the experimental cycle.

High Customization Level

We can tailor engineering solutions based on the client's provided strain type and research goals (e.g., specific carbon source adaptation, purity enhancement) , meeting differentiated needs.

Expert Analysis Support

Experimental reports include data interpretation and mechanism analysis , helping clients understand the underlying logic of strain performance changes and providing direction for subsequent research.

We are dedicated to providing solid experimental foundations and technical support for enhancing PHA strain performance through scientific and rigorous methods.

Process

CD Biosynsis's PHA strain engineering service follows a standardized research workflow, ensuring every step is precise and controllable:

• Requirement Alignment and Baseline Analysis: Thoroughly understand the client's strain background, current performance metrics, and engineering objectives. Collect the strain's foundational data (e.g., carbon source utilization type, current yield) to define the optimization direction.
• Technical Solution Design: Based on the strain's characteristics and engineering goals, design gene editing targets, metabolic pathway adjustment plans, and experimental validation methods , forming a detailed Strain Engineering Research Protocol.
• Strain Editing and Construction: Complete gene editing vector construction, strain transformation, and screening in the laboratory. Verify editing accuracy using molecular biology techniques to obtain the engineered strain.
• Performance Validation Experiments: Conduct comparative fermentation experiments under laboratory conditions , controlling key parameters , and measuring the difference between the engineered strain and the wild-type strain in terms of yield, purity, and carbon source utilization rate.
• Result Report Output: Organize the experimental data and compile a Strain Engineering Experimental Report that includes data charts, result analysis, and technical summary , providing objective performance assessment and subsequent recommendations.

Technical communication with the client is maintained throughout the process , with timely feedback on research progress and key findings.

Discover an effective path to PHA strain performance breakthroughs, starting with professional engineering services! CD Biosynsis provides customized PHA strain engineering solutions:

• PHA Strain Engineering Technical Manual , including common engineering strategies and experimental method references.
• Contracted clients enjoy discounted strain genetic background analysis, providing precise basis for solution design.
• Experimental reports include complete raw data , supporting subsequent research traceability and validation.