CD Biosynsis offers integrated Metabolic Engineering and Pathway Design Services, providing a comprehensive solution to rationally optimize biological systems for enhanced bioproduction. Our service spans the entire Design-Build-Test-Learn (DBTL) cycle, from the in silico design of novel biochemical pathways to the construction of highly efficient engineered strains. By leveraging computational tools, synthetic biology techniques, and advanced genetic engineering, we enable the sustainable, high-yield manufacturing of chemicals, fuels, materials, and therapeutics across various host organisms. We are your partner in transforming challenging biochemical processes into commercially viable biomanufacturing solutions.
Get a QuoteMetabolic Engineering involves the targeted modification of an organism's metabolism to direct carbon flux toward a desired product. Our integrated approach starts with Pathway Design, where computational modeling identifies optimal enzymatic routes and genetic targets (knockouts, overexpression, pathway tuning). This is followed by strain construction using precision tools like CRISPR and synthetic circuits, ensuring the final biological system is not only high-yielding but also stable and robust for industrial scaling. This combination of rational design and precise engineering sets the foundation for advanced biomanufacturing.
Computational prediction of optimal pathways and identification of metabolic bottlenecks using Flux Analysis.
Implementation of rational strategies (knockout/overexpression/tuning) to optimize product yield and purity.
Building synthetic circuits (switches, logic) for precise, dynamic control over pathway function and cell behavior.
Access our full range of solutions tailored to different host organisms and cellular systems.
Targeted optimization of bacteria (E. coli, Yeast) for high-efficiency biosynthesis.
Harnessing microalgae for sustainable production of biofuels and high-value lipids.
Engineering plants for improved yield of natural products and enhanced nutrition.
Optimization of mammalian cells (CHO, HEK) for therapeutic protein production.
Simplified, open systems for rapid prototyping and synthesis of complex molecules.
A categorized list of all DNA manipulation and control techniques we employ in our projects.
Our systematic workflow ensures high efficiency and rational decision-making at every stage of the project.
Design & Modeling
Build & Engineering
Test & Analysis
Learn & Optimization
Define project goal, target compound, and host organism.
Pathway Design: Computational analysis, FBA, and thermodynamic assessment to determine optimal gene targets and metabolic modifications.
Design of genetic parts and control circuits.
Utilize automated synthesis and assembly platforms for DNA constructs.
Precision genetic manipulation (CRISPR/Cas, Transposons) for stable genomic integration of pathways and circuits.
Chassis Optimization to prepare the host for high-flux metabolism.
Data analysis informs the iterative refinement of the pathway and control logic.
Fine-tuning gene expression using promoter libraries (Promoter Engineering).
Final delivery of the fully optimized, robust strain and comprehensive documentation.
Rational Design & In Silico Modeling
We start with computational tools to predict the most effective pathway modifications, minimizing costly and time-consuming experimental cycles.
Full DBTL Cycle Execution
We manage the entire process from concept design to final strain optimization, ensuring smooth transitions and comprehensive quality control.
Integration of Circuit Control
Our unique expertise in Genetic Circuit Engineering enables dynamic control over production, enhancing yield and mitigating pathway toxicity.
Cross-Organism Scalability
Solutions are developed with industrial scale-up in mind, providing strains optimized for robust and high-titer performance across diverse hosts.
"The integrated pathway design and optimization strategy was excellent. The initial FBA modeling accurately predicted the knockout targets, leading to a 4-fold titer increase in our E. coli strain faster than we anticipated."
Dr. Samuel Chen, CTO, BioMaterials Innovation Inc.
"By combining their Chassis Optimization service with a simple Genetic Circuit, CD Biosynsis solved the product toxicity issue that had halted our scale-up efforts for over a year. The resultant strain is exceptionally stable."
Ms. Laura Varga, Process Engineering Lead
"Their expertise in engineering non-model organisms, specifically an algal strain, allowed us to successfully introduce and optimize a complex polyketide pathway. The documentation provided for the DBTL cycle was highly valuable."
Prof. John Davies, Marine Biotechnology Research Group
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What is the key output of the Pathway Design phase?
The key output is a Detailed Genetic Strategy, which specifies the exact genes to be modified (e.g., knockout, overexpression) and the optimal pathway structure (enzyme order, expression levels) required to achieve the target production yield based on in silico modeling results.
How do you select the best host organism for a project?
Host selection depends on the target product's complexity, required post-translational modifications (favoring eukaryotic cells like yeast or mammalian), scalability needs (favoring E. coli or Yeast), and the complexity of the native metabolic background. We offer consultation to match your product to the ideal host.
Can I provide my own pathway enzymes for the service?
Absolutely. We fully support client-provided enzyme candidates and pathways. We can then focus on optimizing the expression of those parts, fine-tuning their balance (Promoter Engineering), and integrating them stably into the chosen host chassis.
What is the primary role of Genetic Circuit Engineering in a full ME project?
It provides dynamic control. For example, a circuit can be designed to temporarily inhibit cell growth genes while maximizing product synthesis genes only during the production phase, or to activate a detoxification pathway if toxic intermediates build up.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
|
There is no product in your cart. |
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.