CD Biosynsis offers comprehensive Metabolic Engineering Services tailored to optimize microbial and cellular systems for enhanced production of target compounds. By precisely manipulating biosynthetic pathways in diverse host organisms—including bacteria, yeast, fungi, algae, and mammalian cells—we help researchers and industry partners achieve high-yield, sustainable biomanufacturing solutions. Our expertise spans from rational design and pathway modeling to high-throughput screening and fermentation optimization, utilizing cutting-edge synthetic biology and gene editing tools. We are committed to accelerating your projects in areas such as natural product synthesis, sustainable fuels, industrial enzymes, and novel therapeutics.
Get a QuoteMetabolic Engineering is a field of synthetic biology and systems biology that focuses on the targeted modification of cellular metabolism to improve product yield, enhance stress tolerance, or introduce novel functionalities. It involves the systematic rewiring of biochemical pathways within an organism using genetic engineering techniques. The process often starts with computational modeling to identify rate-limiting steps and promising genetic targets, followed by the actual modification, and subsequent high-throughput screening and optimization.
Comprehensive strategies for pathway optimization across various prokaryotic hosts for enhanced production.
Tailored genetic modifications for key bacterial species used in industrial biosynthesis.
High-precision engineering of the industry's workhorse for heterologous production.
Optimizing Gram-positive bacteria for robust secretion and large-scale fermentation.
Engineering strains known for their complex metabolic capabilities and environmental tolerance.
Optimization of Corynebacterium strains, primarily for amino acid production and specialty chemicals.
Engineering filamentous fungi for enzyme production and secondary metabolite synthesis.
Leveraging yeast as a platform for producing complex molecules, including drugs and biofuels.
Engineering of the classic budding yeast for optimized chemical and biofuel synthesis.
Specialized for high-yield expression and secretion of heterologous proteins.
Optimization of high-production strains for food ingredients and industrial enzymes.
Pathway optimization for high-value secondary metabolites, like antibiotics.
Enhanced production of cellulases and other important industrial enzymes from Trichoderma.
Optimization of microalgae for biofuel production, high-value lipids, and pharmaceuticals from CO2.
Targeted engineering of a versatile model algal organism for protein and lipid enhancement.
Focusing on highly efficient lipid accumulation and EPA production in Nannochloropsis.
Engineering crop or medicinal plants for enhanced nutritional value or synthesis of specialized metabolites.
Optimization of mammalian cell lines (CHO, HEK) for high-quality therapeutic protein production (e.g., antibodies).
A simplified, open platform for rapid prototyping and biosynthesis without the complexity of a living cell.
Our systematic workflow ensures a highly rational and efficient process from design to implementation and verification.
Design & Analysis
Engineering & Construction
Screening & Optimization
Validation & Delivery
Customers provide the target compound and desired host organism.
Our team performs Pathway Modeling & Flux Analysis to identify metabolic bottlenecks and potential genetic targets (knock-out, knock-in, down-regulation).
A detailed metabolic engineering strategy is formulated based on the analysis.
Utilize advanced synthetic biology tools (CRISPR-Cas, Golden Gate assembly) for high-precision genetic modification.
Construction of plasmids and genetic parts (promoters, terminators, gRNAs).
Transformation and stable integration of the designed metabolic pathway into the host organism.
Provide detailed Analytical Reports including HPLC, GC-MS data on product titer, yield, and purity.
Deliver the optimized, high-performing engineered strain and comprehensive documentation.
Post-delivery technical support for scale-up and further research.
Advanced & Integrated Platform
We integrate CRISPR-Cas technology, AI-driven design, and liquid handling automation for precise and high-throughput strain construction.
Cross-Organism Expertise
Deep knowledge in engineering diverse hosts, from E. coli and Yeast to Algae and CHO cells, ensuring the best platform for your specific product.
Customized Design-Build-Test-Learn
Our DBTL cycle is highly flexible, adapting our strategies to overcome unique metabolic challenges and optimize yield and purity.
Scalability and Industrial Readiness
Strains are optimized with industrial applications in mind, facilitating a smooth transition from lab bench to large-scale bioreactor production.
"CD Biosynsis successfully optimized our E. coli strain, increasing the titer of our target compound by 300\% in just three months. Their rational design approach, combined with high-throughput screening, was truly impressive. They turned a challenging metabolic pathway into a commercially viable process."
Dr. Sarah Johnson, CTO, BioFuel Solutions Inc.
"We tasked them with engineering Pichia pastoris for a complex therapeutic protein. The result was a stable, high-yield strain that met all our quality specifications. The team's expertise in eukaryotic metabolism made all the difference. Highly professional and effective."
Dr. Chen Wei, Research Director, Pharma R\&D Institute
"Their work on Aspergillus metabolism allowed us to significantly reduce production costs for a key industrial enzyme. The project was delivered on time, and the quality of the technical data provided was excellent, allowing for immediate process scale-up."
Mr. David Lee, Process Engineering Manager, Global Biotech Company
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
Which host organism is best for my compound?
The ideal host depends on the complexity of your target compound. For simple molecules and high yield, E. coli or Yeast (S. cerevisiae) are common. For complex eukaryotic proteins, Pichia pastoris or mammalian cells (CHO) are often preferred. We conduct a pre-project consultation to recommend the most suitable host based on pathway complexity, desired post-translational modifications, and scalability goals.
How do you ensure the stability of the engineered strain?
Strain stability is crucial. We employ strategies such as genomic integration (instead of plasmid-based expression), use of strong and stable promoters, and applying Adaptive Laboratory Evolution (ALE) under selective pressure to ensure the engineered traits remain stable over multiple generations, especially under fermentation conditions.
What is the typical turnaround time for a metabolic engineering project?
A full Design-Build-Test-Learn cycle usually takes 3 to 6 months, varying significantly based on the project's complexity, the number of genetic targets, and the target yield increase. Simple modifications may take less, while de novo pathway introduction and optimization may take longer.
Can you work with non-model organisms or newly sequenced microbes?
Yes. Our platform is adaptable. We offer services to establish genetic tools and engineering protocols for non-model organisms, leveraging our expertise in comparative genomics and CRISPR-Cas system transfer to new hosts.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What kind of data do you provide upon project completion?
Upon completion, we deliver the final optimized strain, a comprehensive technical report including the entire engineering strategy, QC data (sequencing, PCR verification), and detailed analytical data (e.g., HPLC, GC-MS) quantifying the product titer, yield, and purity.
Does Metabolic Engineering allow customisability?
Yes, every metabolic engineering project is inherently customized. We tailor the entire DBTL cycle—from computational modeling and pathway design to host selection and fermentation protocols—to meet your specific compound and production goals.
What is the process for keeping data private and confidential?
We adhere to strict data privacy policies. All customer data, proprietary pathways, and experimental results are treated with the highest level of confidentiality and are protected by a Non-Disclosure Agreement (NDA) signed prior to project commencement.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.