CD Biosynsis offers comprehensive Protein Engineering Services to design, evolve, produce, and characterize proteins with novel or enhanced properties. Our integrated platform leverages both rational and directed evolution approaches to tailor proteins for specific applications, including improved catalytic efficiency, enhanced stability, novel binding affinity, and unique functional traits. Whether you need de novo Protein Design, large-scale Recombinant Protein Expression, or precise Protein Modification, our services cover the entire lifecycle of protein development, ensuring high-quality, customized solutions for therapeutics, industrial enzymes, and research reagents.
Get a QuoteProtein Engineering is the science of developing useful or valuable proteins by manipulating their amino acid sequences using recombinant DNA technology. It falls into two main strategies: Rational Design, where knowledge of protein structure and function guides specific modifications, and Directed Evolution, which mimics natural selection in a laboratory setting to screen vast libraries of variants for desired traits. The resulting engineered proteins are critical for modern biotechnology, driving innovations in industrial catalysis, therapeutic biologics, and diagnostics.
Structure-guided and computational methods to introduce specific mutations for altering stability, binding, or specificity.
Creating and screening large mutant libraries to iteratively improve a protein's function (e.g., thermal stability, catalytic activity).
Full-spectrum service utilizing E. coli, Yeast, Insect, and Mammalian systems for optimal production of complex proteins.
Tailored large-scale production, including optimization of fermentation, refolding, and purification strategies for industrial needs.
Rapid prototyping and production of toxic, difficult-to-express proteins in an open, non-living system.
Chemical modification, site-specific conjugation (e.g., PEGylation), and labeling (fluorescent/biotin) for assays and imaging.
Comprehensive analysis of purity, aggregation state (SEC), binding affinity (BLI/SPR), and thermal stability (DSC/DSF).
We execute a streamlined, iterative process to deliver optimized proteins with verified function.
Design & Gene Synthesis
Evolution & Screening
Expression & Purification
Functional Validation
Define target function and initial structure (if known).
Rational Design: In silico modeling and mutation selection.
Directed Evolution: Design of mutagenesis strategy and library creation (random or focused).
Gene synthesis and codon optimization for the chosen host.
High-throughput construction of genetic variants.
Display methods (Phage/Yeast) or automated robotic screening of large libraries.
Identification and selection of lead candidates with improved properties (e.g., higher Kcat, better Kd).
Comprehensive analysis of the purified protein's physiochemical properties.
Functional Assays: Activity assays for enzymes or Binding assays (SPR/BLI) for affinity molecules.
Final report detailing all engineering steps, QC data, and functional characterization results.
Dual-Strategy Expertise
We seamlessly integrate Rational Design and Directed Evolution to tackle even the most challenging protein targets, maximizing success rates.
High-Throughput Screening
Automated platforms allow for the rapid screening of millions of variants, efficiently identifying highly improved candidates from large libraries.
Comprehensive Characterization
Beyond purification, we offer advanced services like BLI/SPR for binding kinetics and DSC for stability, delivering fully characterized proteins.
Specialty Production Options
Access to specialized methods like Cell-Free Expression for difficult targets and Custom Modification for complex labeling requirements.
"We used the Directed Evolution service to improve the thermal stability of an industrial enzyme. Within three iterative rounds, CD Biosynsis delivered a variant with a T_m increase of 15^\circ C and a 30\% increase in turnover rate, a game-changer for our process."
Dr. Kai Zimmer, CTO, Industrial Biotechnology Co.
"The Protein Design team successfully engineered a monomeric fusion protein that was previously aggregating. The final product was highly pure, stable, and functionally active, confirmed by their SEC and SPR characterization data."
Ms. Elena Rodriguez, Lead Biopharma Researcher
"Their Cell-Free Expression platform allowed us to quickly prototype a highly toxic transcriptional regulator that failed in all conventional systems. The rapid turnaround significantly accelerated our assay development phase."
Prof. Alan Thompson, Synthetic Biology PI
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
When should I choose Directed Evolution over Rational Design?
Choose Rational Design when you have detailed structural or mechanistic knowledge of the protein to guide specific mutations. Choose Directed Evolution when the desired function is complex, or when structural knowledge is limited, as it efficiently explores a large sequence space to discover novel solutions.
What is the advantage of Cell-Free Protein Expression?
Cell-free systems offer rapid prototyping (hours vs. days), the ability to easily add non-natural amino acids, and the capability to produce proteins that are toxic to living cells, all in a linear, open reaction format.
What kind of Protein Modification and Labeling services do you offer?
We offer site-specific labeling (e.g., using Sortase or Click chemistry), general chemical labeling (fluorescent dyes, biotin), and bioconjugation strategies such as PEGylation for improved pharmacokinetics of therapeutic proteins.
How do you characterize the binding affinity of engineered antibodies/proteins?
We use state-of-the-art label-free technologies like Bio-Layer Interferometry (BLI) and Surface Plasmon Resonance (SPR) to determine precise kinetic parameters (ka, kd) and equilibrium dissociation constants (Kd), ensuring the engineered affinity meets your specifications.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.