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Trusted by Leading Research & Pharma Institutions

Custom Protein Directed Evolution Services

Accelerate your protein optimization workflow with precision directed evolution solutions. From error-prone PCR to saturation mutagenesis libraries, we deliver expertly designed variant panels with verified diversity to power your enzyme engineering and antibody maturation projects.

NGS Verified
Deep Mutational Scanning
Expert Consultation
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Trusted by leading research and pharmaceutical institutions

MIT
Pfizer
Stanford
Novartis
Johns Hopkins
Roche

Why Choose Us

Comprehensive mutagenesis strategies
Up to billions of variant diversity
Complete coverage guarantee
NGS-verified library quality

Saturation Libraries

Complete AA coverage with NNK/NNS

Error-Prone PCR

Global random mutagenesis

DNA Shuffling

Recombination of beneficial mutations

Library Coverage
>95%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Comprehensive Directed Evolution Solutions

Our platform combines multiple mutagenesis strategies with high-throughput screening capabilities to accelerate your protein engineering research from concept to discovery.

Saturation Mutagenesis

Generate comprehensive single-site or multi-site saturation libraries covering all 20 amino acids at each target position. Our optimized NNK/NNS degenerate codon strategy ensures balanced library representation with minimal bias and maximum functional variant recovery.

  • Complete 19-codon coverage per position
  • Optimized degenerate codon design
  • NGS-verified library uniformity
  • Arrayed or pooled delivery formats

Random Mutagenesis

Error-prone PCR and DNA shuffling technologies enable broad exploration of sequence space for proteins with unknown improvement targets. These global mutagenesis approaches introduce diverse point mutations across entire genes or specific regions.

  • Controlled mutation frequency
  • Full-gene or regional targeting
  • DNA shuffling for recombination
  • High-throughput library construction

Deep Mutational Scanning

Comprehensive variant effect mapping at nucleotide resolution across entire protein domains.

NGS Quality Control

Every library undergoes next-generation sequencing verification with detailed coverage reports.

Flexible Library Types

From single-site saturation to complex combinatorial libraries, tailored to your research goals.

Ready to Evolve Your Protein?

Get a customized quote for your directed evolution project.

Technology Platform

Advanced Mutagenesis Technologies

Industry-leading mutagenesis platforms ensuring high-quality variant libraries for every project.

Synthetic Library Design

Arrayed semiconductor-based DNA synthesis enables precise control over codon usage and amino acid distribution. Every variant is designed with complete user-defined parameters.

Precise Control Zero Bias

Error-Prone PCR

Controlled random mutagenesis using modified polymerases with defined error rates. Ideal for global sequence space exploration when structural information is limited.

Tunable Frequency Full-Gene Coverage

DNA Shuffling

In vitro homologous recombination of gene fragments to combine beneficial mutations from multiple parent sequences. Discovers synergistic epistatic combinations.

Recombination Synergy Discovery

Quality Control

NGS Library coverage quantification
Sanger Individual clone verification
QC Complete QC report included
COA Certificate of Analysis

Library Delivery Formats

Pool Plasmid pool delivery
Array Individual clones in plates
Glycerol Glycerol stocks available
Custom Tailored to your specifications
Specifications

Flexible Options for Diverse Research Needs

Comprehensive specifications to meet your specific directed evolution requirements.

Parameter Saturation Library Random Library Custom Design
Diversity Range Up to 10^9 variants 10^6 - 10^8 variants User-defined
Mutation Strategy NNK/NNS codon design Error-prone PCR/Shuffling Fully tailored
Positions Covered 1-20+ simultaneous sites Full gene or regions As specified
Plasmid Size Up to 15 kb Up to 15 kb Up to 20 kb
Verification NGS + Sanger QC Sanger sampling + QC Custom QC plan
Coverage Guarantee >95% of designed variants Statistical coverage As negotiated
Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

1

Consultation

Project goal definition and strategy selection

2

Design

Library design and codon optimization

3

Construction

Library construction and cloning

4

QC

NGS verification and validation

5

Delivery

Secure packaging with comprehensive report

Applications

Diverse Applications Across Protein Engineering

Our directed evolution services support research and development in multiple fields.

Enzyme Optimization & Engineering

Directed evolution enables systematic exploration of sequence space to identify mutations that improve catalytic activity, thermostability, substrate specificity, or enantioselectivity. Our platform supports comprehensive enzyme engineering campaigns from initial library construction through functional screening.

  • Thermostability enhancement
  • Catalytic efficiency optimization
  • Substrate specificity engineering
  • Enantioselectivity improvement
10^9
Maximum library diversity

Antibody Affinity Maturation

Saturation mutagenesis libraries focused on CDR regions enable systematic exploration of antibody sequence space for affinity maturation. Our high-quality variant libraries accelerate the identification of candidates with improved binding affinity and specificity for therapeutic development.

  • CDR affinity maturation
  • Specificity optimization
  • Humanization support
  • Developability improvement
1000x
Affinity improvement demonstrated

Industrial Biotechnology

Directed evolution of enzymes for industrial applications requires robust variants that perform under demanding process conditions. Our platform generates high-quality mutant libraries optimized for screening under industrial-relevant conditions including extreme pH, temperature, and solvent exposure.

  • Thermostable enzyme engineering
  • Solvent tolerance improvement
  • Detergent resistance engineering
  • pH stability optimization
>95%
Library coverage guarantee
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The saturation mutagenesis library exceeded our expectations. Complete coverage at each CDR position and excellent uniformity enabled rapid affinity maturation. The detailed NGS QC report gave us confidence in proceeding directly to functional screening."

S
Principal Scientist
Biotechnology Company

"We used their error-prone PCR service for our enzyme thermostability project. The controlled mutation frequency and uniform distribution made screening straightforward. Identified three beneficial mutations that improved Tm by 15 degrees."

R
Research Director
Academic Research Institution

"The combinatorial directed evolution campaign identified synergistic mutations that would have been missed by single-site approaches. The library coverage and quality were exceptional throughout the multi-round campaign."

L
Lead Researcher
Pharmaceutical Company
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research in directed evolution and protein engineering.

892 Citations

Directed Evolution: Methodologies and Applications

Wang Y, Xue P, Cao M, et al. Chemical Reviews. 2021.

Comprehensive review of gene diversification strategies, screening/selection methods, and continuous evolution approaches. Highlights representative applications in nucleic acids, proteins, metabolic pathways, and genetic circuits.

View DOI
485 Citations

Low-N Protein Engineering with Data-Efficient Deep Learning

Biswas S, Khimulya G, Alley EC, et al. Nature Methods. 2021.

ML-guided paradigm enabling accurate virtual fitness landscape construction using only 24 functionally tested mutant sequences, screening 10 million sequences computationally.

View DOI
127 Citations

Opportunities and Challenges for Machine Learning-Assisted Enzyme Engineering

Yang J, Li FZ, Arnold FH. ACS Central Science. 2024.

Reviews ML-assisted enzyme engineering opportunities and challenges, covering expression optimization, stability, substrate range, and catalytic efficiency improvements.

View DOI
203 Citations

Machine Learning to Navigate Fitness Landscapes for Protein Engineering

Freschlin CR, Fahlberg SA, Romero PA. Current Opinion in Biotechnology. 2022.

Reviews ML revolutionizing prediction of sequence-structure-function relationships, enabling efficient search of sequence space for valuable proteins.

View DOI
89 Citations

Accelerating Biocatalysis Discovery with Machine Learning

Contributors from Industry Partners. ACS Catalysis. 2023.

Emerging computational tools promise to revolutionize protein engineering for biocatalytic applications, covering AI and ML for tailoring biocatalysis in pharmaceutical industry.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our directed evolution services.

What directed evolution strategies do you offer?
We offer comprehensive directed evolution strategies including: (1) Saturation mutagenesis with NNK/NNS codons for complete amino acid coverage at target positions; (2) Error-prone PCR for random mutagenesis across genes or regions; (3) DNA shuffling for recombination of beneficial mutations from multiple parent sequences; (4) Combinatorial multi-site mutagenesis for exploring epistatic interactions. Our team helps select the optimal strategy based on your protein and engineering goals.
How do you ensure library quality and uniformity?
Every library undergoes comprehensive NGS quality control to verify variant coverage, mutation accuracy, and representation uniformity. For saturation libraries, we use optimized NNK/NNS degenerate codons to minimize amino acid bias and ensure representation of all 20 amino acids. Detailed QC reports document coverage statistics, including the percentage of designed variants present and their relative abundance in the final library.
What library sizes can you generate?
Library size depends on the mutagenesis strategy: Saturation mutagenesis libraries typically generate up to 10^9 variants for multi-position combinatorial designs; Error-prone PCR libraries range from 10^6 to 10^8 transformants; DNA shuffling libraries generate 10^7 to 10^9 recombinants. We calculate the minimum library size required for statistical coverage based on your target diversity and screening capacity.
What's the difference between NNK and NNS codons?
Both NNK and NNS are 32-codon degenerate sequences encoding all 20 amino acids. NNK (N = A/T/C/G, K = G/T) produces one stop codon (TAG), while NNS (N = A/T/C/G, S = G/C) produces no in-frame stop codons. NNS is preferred when maximizing functional variants is critical, as it eliminates the risk of truncated products. Both provide uniform amino acid distribution, though with different codon frequencies.
Can you support iterative directed evolution campaigns?
Yes. We support multi-round directed evolution campaigns where beneficial mutations from initial rounds are combined in subsequent libraries. This iterative approach, guided by screening data, enables accumulation of synergistic mutations and progressive improvement of protein properties. Our team can design combinatorial libraries based on identified hits from previous rounds to explore epistatic combinations.
What delivery formats are available?
We offer flexible delivery formats: (1) Plasmid pool delivery for pooled screening approaches; (2) Arrayed delivery in 96-well or 384-well plates for individual clone screening; (3) Glycerol stocks for long-term storage; (4) Lyophilized plasmid DNA for convenience. All deliveries include freeze-dried materials, detailed QC reports, and sequence documentation.

Ready to Start Your Project?

Get a customized quote for your Protein Directed Evolution project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

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