CD Biosynsis provides professional directed evolution services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our scientists have extensive experience in protein engineering.
Introduction to Directed Evolution
Directed evolution is a cyclic process, which recreates the natural evolutionary process. It involves the generation of gene libraries based on one or more parent gene sequences. Through random mutagenesis and recombination, a large number of artificial mutations are created. High-throughput screening and selection can be used to select improved variants with desired properties, including genetic diversity, binding affinity, catalytic properties, thermal and environmental stability. Directed evolution is the most common approach to circumvent our limited understanding of protein structure-function-dynamics relationships.
The goal of synthetic biology is to design biological entities for a wide range of purposes. Directed evolution is a valuable tool for rapidly screening and selecting variant libraries with desired properties. It turns out to be crucial to the success of many synthetic biology efforts. Recent advances in intelligent library design have also helped mitigate the inherent limitations of directed evolution in the scale and rate of library preparation.
Figure 1. Applications of directed evolution and synthetic biology on a systems level. (Bassalo M C, et al., 2016)
Service Offering
CD Biosynsis provides comprehensive services for all stages of directed evolution, including but not limited to library construction, the development of screening and selection platform, and the development of high-throughput assays.
Random Mutagenesis
Random mutagenesis can be used as a powerful tool to generate proteins, enzymes, metabolic pathways, or even entire genomes with desired or improved properties. CD Biosynsis exploits a wide range of methods, including chemical mutagenesis, mutator strains, ep-PCR, ISM, SeSaM, etc., to help meet the specific requirements of our customers. The mutant products will be cloned into the selected specific vectors.
Recombination
Recombination is another widely used method for directed evolution. Based on our advanced technology and rich experiment, CD Biosynsis is here to provide global customers with genetic recombination services using different techniques, including DNA shuffling, StEP, RAISE, RPR, RACHITT, etc.
Mutant Library Construct
Mutant library is a collection of gene variants. The quality of artificially prepared mutant libraries determines the success of directed evolution. CD Biosynsis' advanced high-throughput gene synthesis, mutagenesis, and sequencing platforms enable us to speed up your project by offering mutant library construct services for directed evolution.
High-Throughput Screening
Screening aims to evaluate proteins with the desired property. High-throughput screening methods enable the rapid identification of desirable properties and greatly reduces the chance of missing the desired mutants. By taking advantage of automation, CD Biosynsis can provide customers with high-throughput screening services including FACS, Cell surface display, NMR, HPLC, etc. to satisfy customers' demand.
Protein Selection
Compared with high-throughput screening methods, protein selection can directly eliminate unwanted protein variants by applying certain selection pressure on the mutant library. Therefore, only positive variants can proceed to the next round of directed evolution, making it possible to evaluate a much larger library. CD Biosynsis can provide customers with high-throughput selection services both in vivo and in vitro.
CD Biosynsis has been involved in the field of protein design and engineering for many years. We are fully committed to working with our customers to facilitate the successful completion of their synthetic biology projects.
If you are interested in our services, please contact us for more details.
References
- Bassalo M C, et al. Directed evolution and synthetic biology applications to microbial systems. Current opinion in biotechnology, 2016, 39: 126-133.
- Cobb R E, et al. Directed evolution: an evolving and enabling synthetic biology tool. Current opinion in chemical biology, 2012, 16(3-4): 285-291.
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