CO2 Bio-Fixation Strain Engineering Service for Carbon Capture and Utilization (CCU)

CO2 Bio-Fixation Strains (phototrophs and chemotrophs) offer a sustainable, biological route for Carbon Capture and Utilization (CCU), converting industrial CO2 into high-value chemicals, fuels, and materials. However, commercialization is severely limited by the low efficiency of the native CO2 fixation enzyme (RuBisCO) and the high operational costs associated with light energy or specialized electron donors.

We provide advanced Synthetic Biology and Metabolic Engineering services to overcome these constraints. Our core strategy involves introducing non-native, highly efficient CO2 fixation pathways (e.g., the CETCH cycle) into industrial hosts like E. coli for chemotrophic conversion. Additionally, we engineer photosynthetic hosts (like Cyanobacteria) to directly secrete complex, high-value products (e.g., Isoprenoids) from CO2, ensuring high efficiency and cost-effective conversion of carbon emissions into marketable goods.

Pain Points

The large-scale biological fixation of CO2 faces several critical hurdles:

• Low Fixation Efficiency (RuBisCO): The primary native CO2 fixation enzyme, RuBisCO, is notoriously slow and exhibits a wasteful side reaction with oxygen (photorespiration), significantly reducing carbon utilization efficiency.
• High Energy Cost: For phototrophic systems (Cyanobacteria, algae), the cost of providing adequate light over large reactor areas is a major economic barrier. For chemotrophs, the electron donors (e.g., H2, formate) can be expensive.
• Product Toxicity: Intracellular accumulation of synthesized products in phototrophs can be toxic, limiting the final titer and requiring energy-intensive cell lysis for product recovery.
• Limited Product Spectrum: Wild-type fixation is geared toward biomass accumulation (sugars), limiting the range of high-value products that can be synthesized directly from CO2.

Commercialization requires moving beyond the efficiency limits of native pathways and reducing energy input costs.

Solutions

We deploy advanced Synthetic Biology to create highly efficient CO2-utilizing platforms:

Synthetic CO2 Fixation Pathway Introduction

     

Introduce highly efficient, non-native fixation pathways (e.g., CETCH cycle) into industrial chemotrophic hosts (e.g., E. coli) for CO2 conversion without relying on light.

Direct Secretion Engineering in Phototrophs

Engineer Cyanobacteria to actively secrete high-value products (e.g., Isoprenoids, fuels) directly into the medium, eliminating biomass harvesting and cell lysis costs.

Product Pathway Integration

Integrate synthetic pathways for high-value targets (e.g., Isoprenoids, fatty alcohols) directly downstream of the CO2 fixation pathway for single-step bioconversion.

Energy Source Optimization

Optimize chemotrophic hosts to efficiently utilize cheap, low-carbon electron donors (e.g., industrial waste gases) or enhance light harvesting components in phototrophs.

Our systematic strategy enables high-efficiency conversion of waste CO2 into profitable chemical products.

Advantages

Our CO2 Bio-Fixation Engineering service offers the following key benefits:

High Carbon Fixation Efficiency

Synthetic pathways like CETCH bypass RuBisCO's limitations, achieving higher theoretical and actual CO2 fixation rates.

Reduced Recovery Costs

Direct product secretion eliminates the need for expensive and energy-intensive cell harvesting and lysis for product recovery.

Utilization of Waste CO2

Turns an industrial pollutant (CO2) into a valuable, low-cost raw material, promoting a circular economy model.

Synthesis of Complex Molecules

Engineered hosts can directly synthesize complex, high-value molecules (e.g., Isoprenoids) from a simple C1 source (CO2).

Low Energy Donor Dependency

Optimization allows for utilization of cheaper energy sources (e.g., formate, waste hydrogen), lowering the overall operational expense.

We provide a specialized platform for the sustainable and highly efficient biological conversion of industrial CO2.

Process

Our CO2 Bio-Fixation Engineering service follows a rigorous, multi-stage research workflow:

• Host Selection and Pathway Design: Select the appropriate host (E. coli, Cyanobacteria) and design the synthetic CO2 fixation and product synthesis pathways (e.g., CETCH cycle).
• Synthetic Pathway Construction and Integration: Clone and integrate the complex multi-enzyme pathways into the host genome, optimizing enzyme expression levels for balanced flux.
• Product Secretion/Tolerance Engineering: Engineer membrane transporters for efficient product efflux or enhance host tolerance to high intracellular product concentrations.
• Bioreactor Optimization: Design and test gas-fed bioreactor systems (for chemotrophs) or photobioreactors (for phototrophs), optimizing CO2 concentration, light intensity, and electron donor supply.
• Conversion Rate and Yield Validation: Measure the CO2 uptake rate, product titer, and final carbon yield from CO2 to the target chemical.
• Result Report Output: Deliver a detailed report including engineered strain data, cultivation protocols, and final validated CO2 fixation rate and product yield metrics.

Technical communication is maintained throughout the process, focusing on timely feedback regarding fixation efficiency and product yield.

Explore the potential for sustainable CO2 utilization. We provide customized strain solutions:

• Detailed CO2 Uptake Rate and Product Titer Analysis Report, demonstrating the performance of the engineered fixation pathway.
• Consultation on photobioreactor or gas-fermentation reactor scale-up design.
• Experimental reports include complete raw data on carbon mass balance and product secretion efficiency, essential for economic modeling.