Butanol Bio-Butanol Engineering Service

Butanol (Bio-Butanol) is a key bio-solvent and advanced biofuel with applications in Solvents/Fuels, replacing petroleum-derived alternatives. Its conventional production via ABE Acetone-Butanol-Ethanol fermentation faces severe limitations: ABE fermentation Clostridium is slow, low-titer, and sensitive to product toxicity. This inherent toxicity and poor yield severely hinder its industrial competitiveness.

CD Biosynsis offers a multi-faceted solution centered on strain improvement and process intensification: Metabolic Engineering: Knockout competing pathways e.g., Acetone synthesis in Clostridium or E. coli. This redirects carbon flux directly to Butanol. To overcome cellular toxicity, we Introduce Efflux pumps or membrane engineering to enhance Butanol tolerance and secretion. Finally, we employ Cell-recycle strategies for continuous fermentation to maximize productivity and achieve higher final titers.

Pain Points

The ABE fermentation for Butanol production must overcome these bottlenecks:

• Product Toxicity: Butanol is a solvent that becomes toxic to the producing Clostridium cells at low concentrations typically above 15g/L, limiting the final titer.
• Low Titer and Yield: Due to toxicity and competing pathways, the final Butanol concentration is too low, making downstream recovery by distillation highly energy intensive and expensive.
• Slow Fermentation Rate: Clostridium strains grow and produce Butanol slowly and exhibit a phenomenon called acid crash during the switch to solvent production phase.
• Competing Byproducts: ABE fermentation naturally co-produces Acetone and Ethanol, reducing the selectivity and yield of the target Butanol product.

Industrialization requires increasing Butanol titer and selectivity while improving process speed.

Solutions

CD Biosynsis employs advanced metabolic and process engineering to revitalize Bio-Butanol production:

Flux Redirection Metabolic Engineering

           

We knockout genes for competing pathways e.g. Acetone and acid production in Clostridium or engineer E. coli for maximal Butanol flux.

Enhanced Tolerance and Secretion

We introduce native or synthetic Efflux pumps or modify cell membranes to actively export Butanol and protect the cell from toxicity.

Process Intensification with Cell Recycle

We implement cell-recycle systems e.g. membrane bioreactors for continuous fermentation, allowing the cells to reach and maintain very high densities.

Robust Host Switching E. coli Yeast

For faster growth, we can design a Butanol pathway in robust, high-titer E. coli or Saccharomyces cerevisiae hosts to overcome Clostridium limitations.

Our comprehensive strategy targets both strain robustness and fermentation efficiency for commercially viable Bio-Butanol production.

Advantages

Our Butanol Bio-Butanol engineering service offers these core benefits:

Significantly Higher Titer

Enhanced tolerance and continuous fermentation enables titer increase well above 20g/L, reducing distillation costs.

Increased Product Selectivity

Knockout of Acetone and Ethanol pathways redirects nearly all flux to Butanol, improving yield.

Lower Downstream Cost

Higher Butanol titer means significantly less energy is required for product recovery via distillation or extraction.

Continuous and Stable Production

Cell recycle and improved tolerance allow for long-term high-rate continuous fermentation, boosting productivity.

Flexible Feedstock Utilization

Engineered strains can utilize cheaper and more diverse sugars and lignocellulosic hydrolysates, lowering raw material costs.

We provide the technology necessary to make Bio-Butanol a truly competitive fuel and solvent.

Process

Our Butanol Bio-Butanol engineering service follows a rigorous, multi-stage research workflow:

• Butanol Pathway Engineering: Identify and overexpress Butanol biosynthesis genes in Clostridium or integrate the full pathway into a robust host e.g. E. coli.
• Byproduct Knockout: Utilize CRISPR or other gene editing tools to inactivate competing pathways e.g. Acetone kinase and acid production enzymes.
• Tolerance and Secretion Optimization: Engineer efflux pump expression levels and modify membrane fatty acid composition for increased Butanol tolerance and export.
• Continuous Fermentation System Design: Optimize cell-recycle strategies e.g. cross-flow membrane filtration to maintain high cell density and productivity in a continuous bioreactor.
• Process Validation: Perform long-term continuous fermentation runs and analyze Butanol titer and productivity to confirm performance goals.

Technical communication is maintained throughout the process, focusing on timely feedback regarding yield and product tolerance attributes.

Explore the potential for a high-titer, continuous Bio-Butanol supply. CD Biosynsis provides customized strain and process engineering solutions:

• Detailed Butanol Titer and Productivity Reports g/L, g/L/h from continuous fermentation.
• Consultation on downstream product recovery cost savings based on higher titer.
• Experimental reports include complete raw data on efflux pump expression, Butanol tolerance testing, and metabolic flux analysis.