MANEA Knockout cell line (293T)

Catalog Number: KOA01115

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Specifications

Product Information
Product Name	MANEA Knockout cell line (293T)
specification	1*10^6
Storage and transportation	Shipped on dry ice; Store in liquid nitrogen
Cell morphology	Epithelial-like, adherent
Passage ratio	1:3~1:6
species	Human
Gene	MANEA
Gene ID	79694
Build method	Electroporation/Lentivirus
Mycoplasma testing	negative
Cultivation system	90% DMEM+10%FBS
Parental Cell Line	293T
Quality Control	Genotype: MANEA Knockout cell line (293T)>95% viability before freezing. All cells were tested and found to be free of bacterial, viruses,mycoplasma and other toxins.

Gene Information
Gene Official Full Name	mannosidase endo-alphaprovided by HGNC
Also known as	ENDO; hEndo
Gene Description	N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008]
Expression	Ubiquitous expression in urinary bladder (RPKM 7.3), thyroid (RPKM 6.8) and 25 other tissues See more

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