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MAP3K4 Knockout Cell Lines

Gene: MAP3K4

Official Full Name: mitogen-activated protein kinase kinase kinase 4provided by HGNC

Gene Summary: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO10682 MAP3K4 Knockout cell line (HeLa) Human MAP3K4 1:3~1:6 Negative Online Inquiry
KO10683 MAP3K4 Knockout cell line (HCT 116) Human MAP3K4 1:2~1:4 Negative Online Inquiry
KO10684 MAP3K4 Knockout cell line (HEK293) Human MAP3K4 1:3~1:6 Negative Online Inquiry
KO10685 MAP3K4 Knockout cell line (A549) Human MAP3K4 1:3~1:4 Negative Online Inquiry

Background

MAP3K4 Gene Knockout Cell Lines are specifically engineered cellular models that have had the MAP3K4 gene inactivated using advanced molecular techniques such as CRISPR-Cas9. The MAP3K4 gene encodes a member of the mitogen-activated protein kinase (MAPK) signaling pathway, which plays a pivotal role in various physiological responses, including cell proliferation, differentiation, and apoptosis. By knocking out this gene, researchers can investigate the consequences of its absence on cellular function and signaling mechanisms.

These cell lines enable the study of MAP3K4's role in cellular processes and its contribution to various diseases, including cancer and neurodegenerative disorders. By employing these knockout models, scientists can delve into complex pathways mediated by MAP3K4, providing insights that are essential for identifying potential therapeutic targets and interventions. Their utility in both basic research and applied sciences makes them invaluable in exploring how dysregulation of this pathway contributes to pathophysiology.

One of the standout advantages of MAP3K4 Gene Knockout Cell Lines is their specificity and reproducibility, which surpasses traditional methods of gene silencing. This high level of precision enables researchers to obtain clear, unambiguous results regarding gene function and its implications in disease. Additionally, compared to other knockout models, these cell lines facilitate a more efficient and cost-effective approach, as they are ready-to-use, significantly reducing the time required for experimental set-up.

For researchers and clinicians alike, these knockout cell lines represent a powerful tool for advancing our understanding of MAP3K4-related biology and potential treatment strategies. The distinct ability to manipulate gene expression in a controlled manner allows for robust experimentation that is crucial for making significant advances in both innovative therapeutic development and basic biological science.

Our company stands at the forefront of biotechnological innovation, dedicated to providing high-quality biological products that empower researchers and clinicians with the tools they need to succeed in their scientific endeavors. With our expertise in gene-editing technologies and a commitment to excellence, we offer reliable solutions that enhance research capabilities and drive discoveries in the life sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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