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MACF1 Knockout Cell Lines

Gene: Macf1

Official Full Name: microtubule-actin crosslinking factor 1provided by MGI

Gene Summary: Enables ATP hydrolysis activity; actin binding activity; and microtubule binding activity. Involved in several processes, including positive regulation of Wnt signaling pathway; regulation of focal adhesion assembly; and regulation of neuron projection arborization. Acts upstream of or within several processes, including Wnt signaling pathway; mesoderm formation; and post-translational protein targeting to endoplasmic reticulum membrane. Located in cytoplasm; cytoskeleton; and postsynaptic density. Is expressed in several structures, including central nervous system; genitourinary system; limb segment; lung; and skeletal musculature. Human ortholog(s) of this gene implicated in lissencephaly 9 with complex brainstem malformation. Orthologous to human MACF1 (microtubule actin crosslinking factor 1). [provided by Alliance of Genome Resources, Apr 2025]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO07127 MACF1 Knockout cell line (HeLa) Human MACF1 1:3~1:6 Negative Online Inquiry
KO07128 MACF1 Knockout cell line (HCT 116) Human MACF1 1:2~1:4 Negative Online Inquiry
KO07129 MACF1 Knockout cell line (HEK293) Human MACF1 1:3~1:6 Negative Online Inquiry
KO07130 MACF1 Knockout cell line (A549) Human MACF1 1:3~1:4 Negative Online Inquiry

Background

Macf1 Gene Knockout Cell Lines are genetically engineered cell lines in which the Macf1 gene has been systematically disrupted, allowing for comprehensive studies into the functional role of this gene in various biological processes. Macf1, or Microtubule Actin Crosslinking Factor 1, plays a crucial role in cytoskeletal dynamics, cell adhesion, and intracellular signaling, making it an essential focus in cellular biology research.

The key mechanism underlying these knockout cell lines is the use of CRISPR-Cas9 technology, which allows for precise editing of the genome. By introducing double-stranded breaks in the DNA sequence of the Macf1 gene, researchers can observe the resultant phenotypic changes, offering insights into how the absence of this protein affects cellular behavior, such as proliferation, migration, and response to external stimuli. This tool is indispensable for understanding disease mechanisms, including developmental disorders and cancer, where Macf1 may play a significant role.

In both research and clinical settings, Macf1 Gene Knockout Cell Lines contribute substantially to the elucidation of complex biological pathways and the development of therapeutic interventions. These lines are particularly valuable in preclinical studies where the role of Macf1 in tumor progression or metastasis can be dissected. Furthermore, they facilitate drug discovery efforts focused on targeting actin-microtubule interactions.

Compared to traditional knockout models, Macf1 Gene Knockout Cell Lines offer enhanced specificity and faster generation times due to advanced genome editing techniques. This efficiency not only accelerates research timelines but also reduces costs associated with model organism studies. Our product provides a unique advantage by ensuring high reproducibility and reliable outcomes in experimental applications.

For researchers, clinicians, and biotech firms seeking to explore the implications of the Macf1 gene and its associated pathways, our Gene Knockout Cell Lines represent a high-value tool that can lead to significant advancements in understanding and treating diseases. With extensive expertise in genetic engineering and a commitment to scientific rigor, our company stands at the forefront of developing innovative biological products that empower the scientific community.

Please note that all services are for research use only. Not intended for any clinical use.

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