Gene: H3C12
Official Full Name: H3 clustered histone 12provided by HGNC
Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO34241 | H3C12 Knockout cell line (HCT 116) | Human | H3C12 | 1:2~1:4 | Negative | Online Inquiry |
KO34242 | H3C12 Knockout cell line (A549) | Human | H3C12 | 1:3~1:4 | Negative | Online Inquiry |
H3C12 Gene Knockout Cell Lines represent a cutting-edge biological tool developed for precise gene inactivation studies. These cell lines are engineered to silence the expression of the H3C12 gene using CRISPR/Cas9 gene-editing technology, allowing researchers to investigate the functional role of this gene in cellular processes and disease pathways. By generating these knockout lines, scientists can delve deep into the implications of H3C12 deletion in various biological contexts, facilitating an enhanced understanding of gene function and regulation.
The primary mechanism of action for H3C12 Gene Knockout Cell Lines lies in the targeted disruption of the gene by leveraging CRISPR/Cas9. This advanced genome-editing tool utilizes a guide RNA to direct the Cas9 nuclease to the specific gene locus, resulting in double-strand breaks that lead to gene knockout through non-homologous end joining (NHEJ). This precise genomic modification allows for the generation of stable, heterozygous, or homozygous knockout cell lines that are essential for rigorous analyses in molecular and cellular biology.
The scientific importance of H3C12 Gene Knockout Cell Lines shines brightly in various research domains, including cancer biology, developmental biology, and therapeutic research. Their application in preclinical studies can lead to the identification of novel drug targets and biomarkers, thereby accelerating translational research into clinical applications. These knockout cell lines are particularly invaluable for cross-validation studies and drug efficacy assessments.
Compared to other gene-editing methods, H3C12 Gene Knockout Cell Lines offer unparalleled specificity and efficiency. Traditional methods, such as RNA interference, may produce off-target effects or incomplete knockdown, whereas CRISPR/Cas9 provides a robust means to achieve total gene knockout. Moreover, these cell lines can be readily adapted for high-throughput screening in drug discovery and functional genomics.
For researchers and clinicians striving to uncover the intricacies of genetic function, the H3C12 Gene Knockout Cell Lines represent a critical asset that enhances experimental rigor and broadens the scope of potential investigations. Our company's deep expertise in genomic engineering and commitment to innovative biological products ensures that each cell line is validated for performance, providing users with reliable tools for groundbreaking discoveries.
Please note that all services are for research use only. Not intended for any clinical use.
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