Gene: CTSF
Official Full Name: cathepsin Fprovided by HGNC
Gene Summary: Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq, Jul 2008]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO05334 | CTSF Knockout cell line (HeLa) | Human | CTSF | 1:3~1:6 | Negative | Online Inquiry |
KO05335 | CTSF Knockout cell line (A549) | Human | CTSF | 1:3~1:4 | Negative | Online Inquiry |
CTSF Gene Knockout Cell Lines are genetically modified cell lines in which the Cathepsin S gene (CTSF) has been specifically disrupted or eliminated through advanced gene editing techniques such as CRISPR/Cas9. These cell lines serve as a powerful tool for studying the functional role of the CTSF gene, which is associated with various physiological processes including antigen presentation, immune response, and gene regulation.
The primary function of the CTSF gene involves encoding a lysosomal cysteine protease that plays a crucial role in intracellular protein catabolism and the processing of antigens. By creating knockout cell lines, researchers can elucidate the downstream effects of CTSF deficiency, investigate its implications in diseases such as cancer, autoimmune disorders, and neurodegenerative conditions, and evaluate potential therapeutic targets.
The scientific importance of CTSF Gene Knockout Cell Lines extends to both basic and clinical research settings. These cell lines enable precise in vitro studies that contribute to our understanding of molecular mechanisms underpinning immune response and tumor microenvironments. Moreover, they offer a platform for drug screening and potential therapeutic strategies aimed at modulating the activity of CTSF or compensating for its loss.
Compared to traditional methods of gene knockout, our CTSF Gene Knockout Cell Lines are not only more efficient but also provide a stable genetic alteration, ensuring reproducibility of experimental outcomes. This reliability is complemented by a user-friendly format that allows researchers and clinicians to focus on their studies without the added complexity of generating their own knockout models.
The value of CTSF Gene Knockout Cell Lines lies in their ability to accelerate research and development processes across various fields. By providing validated and ready-to-use cell lines, we empower scientists to conduct high-quality experiments that can lead to breakthroughs in understanding disease mechanisms and potential treatments.
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Please note that all services are for research use only. Not intended for any clinical use.
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