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CA12 Knockout Cell Lines

Gene: CA12

Official Full Name: carbonic anhydrase 12provided by HGNC

Gene Summary: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO38726 CA12 Knockout cell line (HeLa) Human CA12 1:3~1:6 Negative Online Inquiry
KO38727 CA12 Knockout cell line (HCT 116) Human CA12 1:2~1:4 Negative Online Inquiry
KO38728 CA12 Knockout cell line (HEK293) Human CA12 1:3~1:6 Negative Online Inquiry
KO38729 CA12 Knockout cell line (A549) Human CA12 1:3~1:4 Negative Online Inquiry

Background

CA12 Gene Knockout Cell Lines represent a cutting-edge tool utilized for research in the field of molecular biology and genetics. These cell lines have been engineered to have the CA12 gene, which encodes for carbonic anhydrase 12, completely disrupted or "knocked out," allowing researchers to study the effects of the absence of this gene on cellular metabolism and physiological processes.

The key mechanism behind the functionality of CA12 Gene Knockout Cell Lines lies in the CRISPR-Cas9 technology, a powerful genome-editing tool that ensures precise editing of targeted DNA sequences. By creating a targeted knockout of the CA12 gene, these cell lines provide insights into the gene's role in processes such as acid-base balance and tumor-related pathways, enabling the study of its implications in conditions like cancer and metabolic disorders.

From a scientific perspective, these cell lines are invaluable in both research and clinical settings. They allow scientists to elucidate the molecular mechanisms underlying CA12 function and its contribution to various diseases, fostering the development of targeted therapies and novel treatment strategies. Their application in high-throughput screening for drug candidate efficacy or investigating therapeutic interventions sets them apart as a vital component in translational medicine.

What distinguishes CA12 Gene Knockout Cell Lines from alternative products is their rigorously validated knockdown efficiency and consistency in experimental results. This reliability is crucial for obtaining reproducible data, allowing researchers to draw accurate conclusions. Furthermore, these cell lines can be easily cultured and integrated into existing laboratory workflows, making them user-friendly for researchers at various levels of expertise.

For researchers and clinicians alike, the value of CA12 Gene Knockout Cell Lines cannot be overstated. They provide a significant opportunity to advance understanding in gene function and are instrumental in the quest for new therapeutic avenues. Our company prides itself on its expertise in gene knockout technologies, ensuring that our products meet the highest standards of quality and reliability, thereby empowering scientific discovery and innovation.

Please note that all services are for research use only. Not intended for any clinical use.

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