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B3GALNT1 Knockout Cell Lines

Gene: B3GALNT1

Official Full Name: beta-1,3-N-acetylgalactosaminyltransferase 1 (Globoside blood group)provided by HGNC

Gene Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all. [provided by RefSeq, Mar 2017]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO33939 B3GALNT1 Knockout cell line (HeLa) Human B3GALNT1 1:3~1:6 Negative Online Inquiry
KO33940 B3GALNT1 Knockout cell line (HCT 116) Human B3GALNT1 1:2~1:4 Negative Online Inquiry

Background

B3GALNT1 Gene Knockout Cell Lines are specialized cell models engineered to specifically disrupt the expression of the B3GALNT1 gene, a pivotal gene involved in glycosylation processes within cells. This gene encodes a glycosyltransferase that catalyzes the addition of specific sugar residues to glycoproteins and glycolipids, influencing cellular interactions, immune responses, and tumor progression.

The primary function of B3GALNT1 is to facilitate the synthesis of glycan structures crucial for proper cell signaling and adhesion. By utilizing CRISPR-Cas9 technology, our B3GALNT1 knockout cell lines effectively exhibit a complete loss of gene function, allowing for in-depth exploration of the biological consequences of diminished B3GALNT1 activity. Researchers can reliably study the impacts of this gene knockout on cellular behavior, glycosylation profiles, and related pathways, facilitating advancements in understanding cancer biology, autoimmune diseases, and infectious diseases.

The scientific significance of these knockout cell lines is profound, providing unparalleled tools for drug discovery and development. Their applications extend beyond basic research, making them invaluable in clinical settings for investigating therapeutic interventions that target glycan-associated diseases. Researchers and clinicians can leverage these cell lines for high-throughput screening, monoclonal antibody development, and elucidation of glycan-mediated cellular functions.

Compared to conventional cell lines, our B3GALNT1 knockout models stand out due to their precise genetic modifications and the resulting clear phenotypic changes. This specificity allows for a more rigorous assessment of experimental outcomes, leading to more reliable data and a faster pathway to clinical relevance.

For researchers seeking innovative tools, our B3GALNT1 Gene Knockout Cell Lines offer a streamlined approach to dissect complex biological networks and contribute to breakthroughs in therapeutic strategies. Grounded in rigorous scientific expertise, our company is dedicated to advancing biological research through high-quality, customizable knockout models that empower the scientific community in their quest for discovery.

Please note that all services are for research use only. Not intended for any clinical use.

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