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ATP6V0A4 Knockout Cell Lines

Gene: ATP6V0A4

Official Full Name: ATPase H+ transporting V0 subunit a4provided by HGNC

Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]

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Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO07361 ATP6V0A4 Knockout cell line (HeLa) Human ATP6V0A4 1:3~1:6 Negative Online Inquiry

Background

ATP6V0A4 Gene Knockout Cell Lines represent a powerful tool for investigating the roles of specific genes in cellular behavior, particularly those involved in lysosomal function and cellular homeostasis. These cell lines are generated through the precise knockout of the ATP6V0A4 gene, which encodes a critical subunit of the V-ATPase complex responsible for acidifying intracellular organelles. The specificity of this knockout enables researchers to manipulate the cellular environment and study the downstream effects of disrupted vesicle transport and membrane dynamics.

The key functions of ATP6V0A4 Gene Knockout Cell Lines center on elucidating the molecular pathways regulated by the ATP6V0A4 gene. When this gene is disrupted, researchers can observe alterations in lysosomal pH and functionality, providing insights into the mechanisms of disease states such as cancer, neurodegenerative disorders, and lysosomal storage diseases. This model allows for a thorough examination of cellular response mechanisms, aiding in the identification of novel therapeutic targets.

These cell lines are of significant scientific importance due to their ability to mimic pathological conditions in human tissues, making them invaluable in both research and clinical settings. Their applications extend from drug development and toxicity testing to the exploration of gene function and cellular physiology. In comparison to conventional cell lines, ATP6V0A4 knockout models offer clearer insights into gene function and cellular processes, presenting enhanced experimental control and specificity.

One of the unique selling points of ATP6V0A4 Gene Knockout Cell Lines lies in their versatility and the extensive validation protocols applied during their development. This ensures robust performance across a wide range of experimental conditions. The precise genetic modification enables researchers to obtain reproducible and reliable results, thereby accelerating the pace of discovery.

For researchers and clinicians focused on the intricate balance of cellular mechanisms, ATP6V0A4 Gene Knockout Cell Lines provide a valuable resource to advance their understanding of gene function and potential therapeutic interventions. Our company specializes in the generation of high-quality biological products, supported by a dedicated team of experts committed to providing innovative solutions that empower scientific discovery and clinical advancements.

Please note that all services are for research use only. Not intended for any clinical use.

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