CD Biosynsis offers comprehensive E. coli Strain Development and Screening Services dedicated to engineering high-performance microbial cell factories. We integrate cutting-edge genomic tools like CRISPR-Cas9 and Multiplex Automated Genome Engineering (MAGE) with high-throughput screening (HTS) platforms to rapidly identify and optimize superior strains. Our expertise covers developing strains for enhanced metabolic flux , improved tolerance to harsh industrial conditions, and superior recombinant protein expression . We manage the entire Design-Build-Test-Learn (DBTL) cycle, delivering robust, production-ready E. coli strains tailored to your specific application.
Get a QuoteDeveloping an industrial-grade E. coli strain for bioproduction or protein manufacturing involves far more than simple gene cloning. It requires systematically tuning numerous cellular processes, including metabolite transport, carbon flux, stress response, and protein folding capacity. Our services provide a holistic approach, starting with strategic genomic modifications to remove bottlenecks and followed by powerful high-throughput screening to identify rare, high-performing variants. This integrated strategy drastically shortens the development timeline compared to traditional, low-throughput methods.
Rational Genome Editing
Precise, targeted modification (knockouts, knock-ins, promoter swaps) using CRISPR/Cas9 or Recombineering to implement metabolic changes.
Multiplex Automated Genome Engineering (MAGE)
Simultaneous introduction of numerous site-specific mutations across the genome to explore vast combinatorial genetic space.
Adaptive Laboratory Evolution (ALE)
Controlled, long-term selection pressure to guide E. coli toward desired phenotypes, such as stress tolerance or utilization of non-native substrates.
High-Throughput Screening (HTS)
Rapid screening of thousands of engineered colonies using automated liquid handling and plate readers for initial performance metrics (growth, fluorescence, titer).
Fluorescence-Activated Cell Sorting (FACS)
Sorting of single cells based on production capacity (linked to a fluorescent reporter) for rapid enrichment of high-titer variants.
Metabolomic Analysis
Quantitative LC-MS or GC-MS analysis of intracellular and extracellular metabolites to pinpoint metabolic bottlenecks and confirm pathway flux.
Product Titer and Yield Enhancement
Engineering to maximize the concentration (titer) and efficiency (yield) of the final target molecule.
Protein Solubility and Folding
Optimization of host chaperones and rare tRNA expression to improve the solubility and proper folding of recombinant proteins.
Stress and Growth Tolerance
Engineering tolerance to high temperature, low pH, osmolality, and product toxicity for robust fermentation performance.
A structured workflow for systematic and accelerated strain optimization.
Design
Build
Test
Learn & Optimize
Computational Modeling: Use FBA and literature review to identify genetic targets (knockouts, insertions) that enhance flux.
Strategy: Determine the most efficient method (CRISPR vs. MAGE vs. ALE) for modification.
High-fidelity synthesis of required DNA parts (genes, promoters, gRNAs).
Implement modifications using precision genomic editing techniques to create engineered strains/libraries.
Analysis: Compare performance data against the original model to identify remaining bottlenecks.
Iteration: Use insights to inform the next Design cycle , refining genetic targets for continuous improvement until the target yield is met.
Rapid Multiplex Engineering (MAGE)
Our MAGE expertise allows simultaneous testing of dozens of genetic changes, dramatically accelerating the optimization process.
High-Throughput Screening Infrastructure
Automated HTS and FACS pipelines handle large strain libraries (>10,000 variants) for robust lead identification.
Integrated Strain Stability & Robustness
Focus on stable, chromosomal pathway integration and tolerance engineering for successful scale-up and fermentation.
Data-Driven DBTL Cycles
Every decision is informed by quantitative analytical data (metabolomics, titer) for efficient optimization.
"Using their MAGE and HTS platform , we were able to screen hundreds of promoter combinations within a month, achieving a 4x yield increase for our target chemical, which was a huge leap forward."
Dr. Sarah Chen, CTO, Industrial Biotechnology Startup
"The Adaptive Laboratory Evolution service successfully generated an E. coli strain capable of utilizing a cheap, non-native carbon source, drastically lowering our production costs."
Mr. James Porter, VP of Process Development, Biofuels
"Their FACS screening expertise allowed us to isolate a highly productive single-cell clone from a complex library that showed 30\% higher fluorescence than the bulk population."
Ms. Nina Sharma, Research Lead, Protein Expression
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What is the difference between MAGE and CRISPR in strain development?
CRISPR is ideal for precise, targeted edits (single-gene knockout or insertion) based on rational design. MAGE is for highly combinatorial, simultaneous introduction of many short mutations across many genetic loci, often used to rapidly optimize gene expression or ribosome binding sites.
Can you screen for tolerance to specific industrial inhibitors?
Yes. We can design screening assays (often using HTS in media supplemented with the inhibitor) or utilize Adaptive Laboratory Evolution (ALE) to guide the strain toward resistance to harsh conditions, such as high ethanol concentration or inhibitory byproducts.
How large of a strain library can you handle in the HTS pipeline?
Our automated HTS pipeline can routinely screen up to 10,000 to 50,000 individual colonies or variants per campaign, significantly increasing the probability of finding a high-performing strain.
What information is provided for the final optimized strain?
The final delivery includes the stable E. coli strain (glycerol stock), a detailed DBTL report, full Sanger sequencing confirmation of the modified genomic regions, and quantitative performance data (titer, yield) from fermentation validation studies.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.