CD Biosynsis offers specialized and highly efficient E. coli Genome Editing Services to facilitate research in synthetic biology, metabolic engineering, and protein expression. Leveraging advanced techniques like CRISPR-Cas9 and Lambda Red Recombineering (Red/ET), we provide precise genetic modification in various E. coli strains, including K-12, B strains, and common expression hosts. Our services span from single base-pair point mutations and gene knockouts/knock-ins to complex genomic reorganizations and pathway optimization. We guarantee 100% sequence verification for all edited strains, delivering ready-to-use engineered E. coli for your next breakthrough.
Get a QuoteE. coli remains the most widely used host organism for gene expression and metabolic pathway engineering. Our core expertise lies in achieving precise, scarless, and efficient genomic changes that are challenging with traditional methods. We customize our approach based on the target strain and modification complexity, utilizing robust technologies to ensure high editing efficiency and minimal off-target events. This platform is ideal for accelerating projects aimed at enhanced protein yield, novel chemical production, and biological circuit construction.
Gene Knockout
Precise, permanent deletion of target genes (coding or non-coding) using CRISPR or Red/ET for functional analysis and simplified metabolic backgrounds.
Gene Knock-in & Insertion
Integration of foreign genes, promoters, or tags (e.g., His-Tag, fluorescent proteins) into specific genomic loci with scarless insertion.
Point Mutation & Allele Replacement
Introduction of single base-pair changes (SNPs) or short sequence changes for protein engineering and promoter tuning studies.
Metabolic Pathway Construction
Sequential knock-in of multiple genes to assemble novel metabolic pathways for the production of target molecules (e.g., biofuels, pharmaceuticals).
Promoter and Terminator Swaps
Precise replacement of endogenous regulatory elements to optimize gene expression levels and improve product yield.
Large Fragment Deletion/Rearrangement
Editing to remove large genomic regions or invert/transpose DNA fragments for significant streamlining of the host genome.
Expression Strain Optimization
Optimization of E. coli expression strains (e.g., BL21(DE3) derivatives) for enhanced solubility or increased yields of difficult-to-express proteins.
Genome Minimization
Systematic removal of non-essential genes to create a reduced genome host, improving stability and predictability.
Multiplex Editing (MAGE)
Simultaneous introduction of multiple precise mutations across the genome to rapidly explore large genetic landscapes.
A proven, high-throughput pipeline for reliable and validated strain delivery.
Design & Strategy
Editing & Selection
QC & Validation
Delivery
Consultation: Define target modifications, desired strain background, and project goals (e.g., production titer).
Design: Design editing tools (gRNA constructs for CRISPR or homology arms for Red/ET) to ensure precise, scarless editing.
Perform genomic editing using the chosen highly efficient system (CRISPR-Cas9, Red/ET, or Red-Red Recombineering).
Apply stringent selection and counter-selection methods to isolate correctly edited single clones.
Delivery of the final engineered E. coli strain in a standard format (e.g., glycerol stock).
Comprehensive project report including the full editing strategy, sequencing data (Sanger files), and clone storage information.
Scarless & Precise Editing
We ensure genomic modifications are exact, leaving no residual selection markers or unwanted sequences.
Dual Technology Platform
Expertise in both CRISPR-Cas9 for rapid editing and Red/ET Recombineering for maximum flexibility in large insertions/deletions.
100% Sequence Verification
Every single edited strain is confirmed via Sanger Sequencing of the modified locus to guarantee accuracy.
High-Throughput Strain Screening
Capable of handling multiplex editing (MAGE) and screening multiple targets simultaneously to accelerate metabolic engineering efforts.
"We required a 15 kb biosynthetic pathway to be inserted into the E. coli genome. The Red/ET Recombineering service provided a scarless insertion, and the resulting strain showed excellent stability and production."
Dr. Chen Li, PI, Metabolic Engineering Group
"The team successfully performed a complex, five-site Multiplex Point Mutation project on our BL21 expression host, drastically improving the solubility of our recombinant protein. Excellent QC documentation."
Mr. David Smith, Lead Engineer, Bio-Pharma Production
"Their Gene Knockout service for a non-K12 lab strain was rapid and effective. The 100% sequencing confirmation made downstream troubleshooting much simpler."
Ms. Anya Sharma, Research Scientist, Synthetic Biology
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
Which E. coli strains can you edit?
We routinely work with most common lab and industrial strains, including K-12 derivatives (e.g., MG1655, DH5a), BL21(DE3) and its derivatives, as well as various B strains and custom industrial strains. Please inquire about non-standard strains.
What is the difference between CRISPR-Cas9 and Recombineering?
Recombineering (Lambda Red/ET) is excellent for precise, scarless insertions or deletions, particularly for large fragments (>2kb). CRISPR-Cas9 offers higher throughput and efficiency for smaller, targeted changes like point mutations or short knockouts, and can be easily multiplexed.
How do you guarantee that the editing is scarless?
We employ counter-selection markers (e.g., sacB) or temperature-sensitive plasmids that allow the removal of the editing tools and selection markers after the successful modification has occurred, leaving only the desired, scarless genomic change.
Can you perform multiplex editing (multiple changes at once)?
Yes, we offer Multiplex Automated Genome Engineering (MAGE) services and multiplex CRISPR approaches to introduce multiple mutations simultaneously, drastically speeding up the optimization of complex metabolic pathways.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.