CD Biosynsis provides expert E. coli Synthetic Biology and Metabolic Engineering Services , enabling the customized design and optimization of biological systems for enhanced biological production. We use rational design principles combined with high-throughput methodologies (CRISPR/Cas9, Red/ET Recombineering) to rewire E. coli metabolism. Our comprehensive services include the assembly of novel biosynthetic pathways , host strain engineering for improved tolerance and yield, and the implementation of genetic circuits to control expression. We help researchers and industry partners accelerate the development of sustainable production methods for target chemicals, fuels, and pharmaceuticals.
Get a QuoteMetabolic engineering in E. coli requires precise, multi-step genomic modifications to maximize the flux toward a desired product while minimizing energy wastage and byproduct formation. Our synthetic biology approach treats the E. coli cell as a customizable chassis, allowing us to implement and fine-tune complex pathways that may include dozens of foreign enzymes. We focus on integrating entire biosynthetic pathways into the host genome for stability and utilize Design-Build-Test-Learn (DBTL) cycles to rapidly iterate and optimize production strains for industrial scalability.
Pathway Design and Modeling
In silico prediction and FBA (Flux Balance Analysis) modeling to design the most efficient metabolic route for target compound production.
Pathway Component Synthesis & Assembly
Synthesis and assembly of multiple foreign genes into operable long DNA cassettes for chromosomal or plasmid integration.
Refactoring and Codon Optimization
Optimization of GC content, codon usage, and promoter sequences for high, balanced expression of pathway enzymes in the E. coli host.
Nativel Pathway Knockout
Targeted deletion of native competitive pathways (e.g., acetate or lactate production) to redirect carbon flux towards the engineered product.
Genomic Integration
Stable, scarless insertion of large biosynthetic pathways into the E. coli chromosome for robust, long-term expression.
Host Tolerance Engineering
Enhancing resistance to product toxicity, high osmotic pressure, or pH stress to improve cell viability and overall fermentation yield.
Dynamic Pathway Control
Implementation of responsive genetic sensors and circuits to dynamically regulate gene expression based on metabolite levels or environmental cues.
Combinatorial Pathway Libraries
High-throughput construction of libraries varying promoter strength, RBS sequences, and enzyme variants to find optimal combinations.
MAGE and PACE Screening
Utilizing MAGE (Multiplex Automated Genome Engineering) and PACE for rapid, in vivo directed evolution and selection of high-producing strains.
A systematic and iterative approach to rapidly optimize production strains.
Design & Strategy
Build & Assembly
Test & Quantification
Learn & Optimization
Design: Computational modeling to predict optimal gene targets, flux distributions, and enzyme combinations.
Strategy: Define the expression host, vector type, and regulatory elements needed for target production.
High-fidelity synthesis of entire pathways and component optimization (e.g., codon optimization).
Precise genomic editing (CRISPR/Red/ET) for scarless modification and pathway integration.
Data Analysis: Use quantitative data to refine the in silico model and identify rate-limiting steps or bottlenecks.
Re-design: Initiate the next optimization cycle (e.g., enzyme evolution or promoter tuning) based on the collected performance data.
Integrated DBTL Platform
Rapid, iterative optimization cycle ensuring the fastest route from design to high-performing production strain.
Complex Pathway Integration
Expertise in assembling and chromosomally integrating multi-gene, long-chain biosynthetic pathways for stable expression.
Advanced Genomic Tools
Use of MAGE, CRISPR screening, and targeted editing to explore vast genetic space for optimal strain performance.
Custom Strain Backgrounds
Ability to work with both standard (K-12, BL21) and customer-proprietary E. coli strains for industrial relevance.
"CD Biosynsis successfully engineered our E. coli host strain, achieving a 10x increase in the yield of our target terpenoid. The genomic integration and Pathway Refactoring resulted in excellent stability under fermentation conditions."
Dr. Marcus Lee, CEO, Sustainable Chemical Production
"The MAGE screening approach allowed us to rapidly test thousands of combinations for promoter and RBS strength within our multi-enzyme pathway, significantly reducing our optimization timeline."
Ms. Elena Vasquez, Lead Scientist, Biofuel Development
"Their Dynamic Pathway Control circuit successfully implemented an auto-regulatory switch in our strain, preventing product toxicity at high concentrations and boosting the final titer."
Dr. Kenji Tanaka, PI, Advanced Synthetic Biology
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What kind of products can you help us synthesize?
We engineer E. coli for the production of diverse high-value molecules, including natural products (e.g., terpenes, polyketides), amino acids, small molecule pharmaceuticals, bioplastics, and platform chemicals/biofuels.
How long does the typical optimization cycle (DBTL) take?
A typical Design-Build-Test-Learn cycle often ranges from 4 to 8 weeks, depending on the complexity of the pathway and the number of genomic targets. Our MAGE technology accelerates the Build and Test phases significantly.
What is MAGE and how is it used in E. coli engineering?
MAGE (Multiplex Automated Genome Engineering) is a powerful method used to rapidly and simultaneously introduce multiple precise point mutations or short sequence changes across the E. coli genome, which is crucial for combinatorial pathway optimization.
Can you integrate a pathway of 10 or more genes?
Yes. We specialize in assembling large, multi-gene pathways (up to 20kb) and integrating them stably into the E. coli chromosome using Red/ET Recombineering or iterative CRISPR methods.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.