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MCFD2 Knockout Cell Lines

Gene: MCFD2

Official Full Name: multiple coagulation factor deficiency 2, ER cargo receptor complex subunitprovided by HGNC

Gene Summary: This gene encodes a soluble luminal protein with two calmodulin-like EF-hand motifs at its C-terminus. This protein forms a complex with LMAN1 (lectin mannose binding protein 1; also known as ERGIC-53) that facilitates the transport of coagulation factors V (FV) and VIII (FVIII) from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment (ERGIC). Mutations in this gene cause combined deficiency of FV and FVIII (F5F8D); a rare autosomal recessive bleeding disorder characterized by mild to moderate bleeding and coordinate reduction in plasma FV and FVIII levels. This protein has also been shown to maintain stem cell potential in adult central nervous system and is a marker for testicular germ cell tumors. The 3' UTR of this gene contains a transposon-like human repeat element named 'THE 1'. A processed RNA pseudogene of this gene is on chromosome 6p22.1. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2016]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO02089 MCFD2 Knockout cell line (HeLa) Human MCFD2 1:3~1:6 Negative Online Inquiry
KO02090 MCFD2 Knockout cell line (HCT 116) Human MCFD2 1:2~1:4 Negative Online Inquiry
KO02091 MCFD2 Knockout cell line (HEK293) Human MCFD2 1:3~1:6 Negative Online Inquiry
KO02092 MCFD2 Knockout cell line (A549) Human MCFD2 1:3~1:4 Negative Online Inquiry

Background

MCFD2 Gene Knockout Cell Lines represent a powerful tool in molecular biology research, designed specifically to explore the functional role of the MCFD2 gene. These cell lines have been genetically engineered to knock out the MCFD2 gene, allowing for detailed investigation into its contributions to cellular mechanisms, particularly those related to protein trafficking and secretion pathways.

The primary function of MCFD2 involves its role as an adaptor protein that facilitates the transport of specific cargo, including coagulation factors, from the endoplasmic reticulum to the Golgi apparatus. The knockout of this gene disrupts these critical processes, providing a unique opportunity to study the downstream effects on cellular physiology, pathophysiological conditions, and related diseases, such as factor VII deficiency. Researchers can utilize these cell lines to elucidate mechanisms of secretion and identify potential therapeutic targets.

In both research and clinical settings, MCFD2 Gene Knockout Cell Lines stand out due to their ability to model human diseases at a cellular level, enabling the development of targeted therapies and novel treatments. The specificity of these knockout lines allows for high-fidelity experimentation, minimizing background noise often present with other models. Unlike alternatives, which may not fully recapitulate the gene's loss-of-function effects, our cell lines provide a more accurate platform for drug screening and functional assays.

These gene knockout cell lines are invaluable for researchers focused on understanding disease mechanisms, screening for drug candidates, and identifying therapeutic interventions, ultimately driving advancements in personalized medicine. With years of expertise in developing precision biological products, our company is committed to enhancing the capabilities of researchers and clinicians alike through innovative and reliable experimental tools such as the MCFD2 Gene Knockout Cell Lines.

Please note that all services are for research use only. Not intended for any clinical use.

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