CD Biosynsis offers a premier, integrated platform for Nannochloropsis spp. Genome Editing and Metabolic Engineering Solutions, specifically designed to harness the industrial potential of this oleaginous microalga. Renowned for its extraordinary ability to accumulate triacylglycerols (TAG) and high-value eicosapentaenoic acid (EPA), Nannochloropsis species serve as a high-performance eukaryotic chassis for sustainable biofuel production and nutraceutical manufacturing. Our solutions provide the genetic precision required to navigate the unique biological hurdles of these algae, including their robust cell walls and complex epigenetic regulation mechanisms.
Our expert team provides end-to-end support, moving beyond traditional random mutagenesis toward rational, precision-based design. We utilize cutting-edge CRISPR-based tools—including DNA-free Ribonucleoprotein (RNP) delivery and stable episomal systems—to ensure high editing efficiency and genomic stability. By integrating systems biology with advanced metabolic engineering, we empower researchers to redirect carbon flux with unprecedented accuracy. Whether your goal is to optimize carbon partitioning from starch to lipids or to enhance photosynthetic light utilization in high-density cultures, our platform delivers verified monoclonal strains optimized for industrial-scale performance.
Get a QuoteOptimizing Nannochloropsis spp. involves addressing its highly efficient carbon fixation and lipid biosynthetic pathways. Our engineering solutions prioritize the development of high-fidelity strains that allow for the quantitative study of algal physiology. We employ advanced site-specific nucleases to achieve biallelic or haploid modifications, ensuring that the desired traits are fully and stably expressed.
Our metabolic engineering strategy focuses on the rational redistribution of carbon and energy resources. By applying Flux Balance Analysis (FBA) and multi-omics integration, we identify specific genetic targets that, when modified, can redirect photosynthetic energy away from storage carbohydrates toward high-value lipid synthesis. This holistic approach ensures that engineered strains maintain robust growth kinetics while achieving the highest possible specific productivity, making them suitable for both open-pond systems and closed photobioreactor applications.
Optimized CRISPR-Cas9 and Cas12a platforms utilizing RNP delivery to bypass silencing and achieve high-efficiency modifications.
Permanent disruption of target genes via targeted indels to eliminate competitive pathways or study gene function.
Site-specific integration of reporters or metabolic cassettes into validated genomic safe harbors via HDR-mediated repair.
Tunable transcriptional silencing for studying essential genes and balancing branched metabolic networks.
Precise single-nucleotide conversion for targeted protein engineering and "scarless" point mutations.
Coordinated multi-gene engineering to maximize the titer and yield of high-value lipids and pigments.
Simultaneous disruption of redundant gene families to create streamlined algal chassis for industrial use.
High-throughput screening and isolation of algal strains optimized for industrial photobioreactor performance.
Quantitative phenotypic assays integrated with predictive genome-scale metabolic modeling (GEM).
Our integrated technical pipeline ensures precision at every step of the Nannochloropsis spp. engineering process.
1. Computational Design
2. Tool Preparation & Build
3. HTS & Monoclonal Selection
4. Stability & Verification
Establishing target metabolic models and designing codon-optimized components. Designing high-specificity gRNAs to avoid off-target activity in the nuclear and chloroplast genomes.
Synthesis of gRNAs and assembly of DNA-free RNP complexes. Transformation is performed via optimized electroporation or biolistic delivery for organelle integration.
Verification: Genotype confirmation via Sanger or Next-Generation Sequencing (NGS). Phenotypic Analysis: Verification of lipid profiles and long-term stability testing. Delivery of cryopreserved strains.
Precision Haploid Editing
Nannochloropsis species are typically haploid, ensuring that genetic modifications manifest phenotypic changes immediately and clearly without allelic interference.
DNA-Free RNP Delivery
Expertise in RNP delivery prevents foreign DNA integration, resulting in cleaner mutant backgrounds ideal for industrial regulation and safety.
Predictive Efficiency
Our strategies are informed by genome-scale metabolic modeling, reducing trial-and-error and accelerating development timelines for high-lipid strains.
Verified Industrial Stability
Strains undergo rigorous trials over dozens of generations to ensure that the genetic modifications and metabolic phenotypes remain constant.
Technical insights for your Nannochloropsis engineering project.
Contact Us1. How do you address gene silencing in Nannochloropsis?
We utilize DNA-free RNP delivery to avoid triggering host silencing or employ vectors featuring native regulatory elements and optimized codon bias to ensure sustained expression levels.
2. Can you target the chloroplast genome specifically?
Yes, we offer specialized chloroplast engineering protocols using biolistic transformation, which is essential for projects targeting the photosynthetic apparatus.
3. How is the monoclonality of the final strain ensured?
We employ automated single-cell isolation (FACS) followed by selective plating. Every strain is verified as monoclonal through genomic junction analysis and sequencing.
4. Do you provide help with identifying metabolic bottlenecks?
Absolutely. Our modeling services utilize Flux Balance Analysis (FBA) to quantitatively identify nodes that limit the production of target bioproducts like EPA.
5. What is the typical turnaround time for a customized algal strain?
Depending on complexity, projects typically take between 14 to 18 weeks from initial design to the delivery of a verified monoclonal strain.
6. Is it possible to generate marker-free modified strains?
Yes. By utilizing RNP delivery or curable episomal vectors, we can provide modified strains that are free of permanent antibiotic resistance markers.
7. Do you support different Nannochloropsis species?
We have established protocols for the most common industrial species, including N. oceanica and N. gaditana, and can optimize for specific lab strains.
8. What type of reporting is provided with the final product?
We provide a comprehensive technical report including genomic sequencing data, growth curves, and metabolic profiling (e.g., lipid quantification).
Would you like to discuss how our modeling services can identify the best enzymatic targets to maximize EPA yields in your Nannochloropsis strains?
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.