CD Biosynsis offers comprehensive Yeast Synthetic Biology and Metabolic Engineering Services designed to transform yeast, primarily Saccharomyces cerevisiae, into high-performance cell factories for sustainable biomanufacturing. As a premier platform for complex pathway assembly and protein expression, yeast is ideal for producing high-value compounds. We provide an integrated approach covering the entire Design-Build-Test-Learn (DBTL) cycle, utilizing advanced genome editing, high-throughput screening, and AI-guided optimization. Our expertise enables the precise engineering of yeast metabolism to enhance flux toward desired products, minimize byproducts, and improve stress tolerance for industrial scale-up.
Get a QuoteYeast serves as a versatile microbial host, capable of performing complex eukaryotic functions such as proper protein folding and post-translational modifications. Metabolic engineering focuses on rerouting the cell's native biochemistry to maximize the production of a target molecule. Our synthetic biology approach systematizes this process, treating the cell as an engineering problem. We design and assemble entire genetic circuits and pathways, integrate them into the yeast genome, and systematically optimize their performance using cutting-edge screening technologies, ensuring rapid attainment of commercially relevant titers and yields.
De Novo Pathway Design
Computational design of non-native metabolic pathways for target molecules, including enzyme selection, codon optimization, and modular assembly.
Complex DNA Synthesis and Assembly
Synthesis of large DNA fragments or entire chromosomes for pathway construction, often utilizing Yeast Homologous Recombination (HR) for high-efficiency assembly.
Promoter and Terminator Libraries
Creation and utilization of characterized libraries of regulatory elements to finely tune the expression level of every gene in a pathway.
Host Strain Chassis Optimization
Knockout of competing pathways, introduction of push-pull mechanisms, and redirection of native carbon flux toward the desired metabolite precursor.
Subcellular Localization Engineering
Targeting enzymes to specific yeast organelles (e.g., mitochondria, peroxisomes) to enhance local concentration and optimize pathway efficiency.
Transport Engineering
Modifying membrane transporters to increase uptake of feedstock or accelerate export of the final product, preventing cellular toxicity.
High-Throughput Screening (HTS)
Automated screening of large yeast libraries using robotics and biosensors to quickly identify high-performing variants.
Metabolic Profiling and Flux Analysis
Quantitative metabolomics (LC-MS) to identify pathway bottlenecks and measure metabolic flux, guiding the next engineering steps.
Bioreactor Validation
Testing engineered strains in controlled miniature bioreactors to validate performance under simulated industrial conditions.
CRISPR/Cas9 Multiplexing
Efficient simultaneous editing of multiple genomic sites for rapid pathway integration and host chassis modification.
Automated HTS and Biosensors
Robotic systems coupled with yeast-specific fluorescent biosensors for fast, quantitative screening of thousands of clones.
AI-Guided Design (ML/BO)
Machine Learning and Bayesian Optimization to rationally design experimental variants and predict optimal genetic configurations.
Production of Bio-Chemicals
Engineering yeast for the high-yield production of terpenes, fatty acids, alcohols, and polyketides.
Therapeutic Protein Expression
Using yeast to express complex proteins requiring glycosylation or other post-translational modifications, like insulin or vaccines.
Sustainable Bioplastics and Polymers
Metabolically engineering yeast to produce precursors for sustainable polymers and materials.
A closed-loop system for continuous strain improvement.
Design (In Silico)
Build (Genetic Engineering)
Test (HTS & Characterization)
Learn (Optimization & Data)
Pathway Modeling: Define the synthetic pathway and use computational tools (AI/ML, FBA) to select optimal genes and regulatory parts.
Design of Experiments (DoE): Rationally select the best genetic variants to synthesize.
DNA Assembly: Synthesize and assemble all genetic components (genes, promoters) into large pathways.
Yeast Editing: Integrate the pathway into the yeast genome using highly efficient CRISPR/Cas9 methods.
Initial Screen: Use HTS robotics to screen thousands of transformants for basic performance metrics.
Deep Analysis: Validate top strains using LC-MS and metabolic profiling to identify bottlenecks.
Data Feedback: Feed all Test results back into the AI/ML model.
Refined Design: The model suggests refined targets for the next round of Build (e.g., specific promoter adjustments or enzyme mutations).
End-to-End DBTL Solution
A seamless, integrated process from computational design to validated, scale-ready strain.
Predictive Accuracy (AI/ML)
Leveraging machine learning to drastically reduce the number of necessary experimental cycles and increase success probability.
Scarless Pathway Integration
High expertise in marker-free and stable chromosomal integration, critical for industrial IP and regulatory compliance.
Accelerated Titer Improvement
Systematic optimization using omics data quickly resolves bottlenecks, leading to faster increases in final product yield.
"The integrated approach, combining CRISPR editing with metabolic profiling, allowed us to precisely identify and fix the flux bottleneck in our key pathway, resulting in a 40% titer increase."
Dr. Samuel Liu, R&D Manager, Specialty Chemicals
"Their expertise in subcellular localization engineering successfully rerouted our synthesis pathway to the peroxisome, significantly reducing toxicity and improving overall yield."
Ms. Clara Dubois, Lead Bioengineer, Sustainable Materials
"The AI-guided DoE reduced our optimization cycles from five to two. This predictive power saved us months and substantial costs in our synthetic cannabinoid project."
Mr. Ben Jacobs, CTO, Bio-Pharmaceutical Startup
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
Why is yeast a preferred host for complex metabolic engineering?
Yeast is eukaryotic, meaning it can perform complex post-translational modifications (like glycosylation) and compartmentalize pathways within organelles, which is crucial for producing complex natural products or therapeutic proteins.
What is the significance of the DBTL cycle in your service?
DBTL (Design-Build-Test-Learn) is our systematic, iterative framework. It ensures that every engineering cycle is informed by the previous results, moving away from trial-and-error toward a rational, accelerated optimization process.
How do you identify pathway bottlenecks in yeast?
We use quantitative metabolic profiling (LC-MS) to measure intermediate metabolites. A high concentration of an intermediate indicates a bottleneck (a slow enzyme) immediately downstream, directing the next engineering effort.
Can you engineer yeast strains for improved stress tolerance?
Yes. By targeting genes related to cell wall integrity, osmotic pressure, or redox balance, we can engineer yeast for better tolerance to extreme temperatures, pH, or high concentrations of toxic products, which is essential for industrial fermentation.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.