CD Biosynsis offers specialized Yeast Protein Expression and Purification Services , leveraging the eukaryotic advantages of yeast hosts like Pichia pastoris and Saccharomyces cerevisiae. Yeast is a highly efficient system for expressing complex proteins, particularly those requiring post-translational modifications (PTMs) like glycosylation and proper folding, making it ideal for therapeutic proteins, antibodies, and functional enzymes. Our integrated service covers everything from host strain selection and expression vector design to large-scale fermentation and high-purity purification, ensuring the delivery of high-quality, biologically active recombinant protein.
Get a QuoteWhile prokaryotic systems like E. coli are fast, they often fail to produce complex, functional eukaryotic proteins. Yeast systems bridge the gap between bacterial and mammalian hosts. They offer the scalability and high cell density of microbes while providing essential eukaryotic machinery for proper folding, disulfide bond formation, and crucial PTMs, which are vital for biological activity. We focus on optimizing every stage—from selecting strong, inducible promoters (e.g., AOX1 in Pichia) to employing efficient secretion pathways—to maximize the yield and fidelity of your target protein.
Host Strain Selection
Selection between Pichia pastoris (high density, strong promoter) and Saccharomyces cerevisiae (well-characterized genetics) based on protein requirements.
Codon and Vector Optimization
Codon optimization of the target gene for the chosen yeast host and selection of high-copy number or chromosomally integrated vectors.
Secretion Signal Integration
Incorporation of native (e.g., alpha-factor) or engineered secretion signals to facilitate extracellular expression, simplifying downstream purification.
Fed-Batch Fermentation
Utilization of high-density fed-batch fermentation in bioreactors (up to 50L) to achieve maximum cell density and protein expression titers.
Induction Optimization
Fine-tuning induction parameters (e.g., methanol feed rate for Pichia) to maximize soluble and functional protein expression.
Solubility and Folding Screening
Rapid screening of expression conditions to promote proper protein folding and solubility, reducing the formation of inclusion bodies.
Multi-Step Chromatography
Employing custom purification schemes, typically including Affinity (Ni-NTA, Strep-Tactin), Ion Exchange, and Size Exclusion Chromatography (SEC).
Tag Removal
Specific enzymatic cleavage (e.g., TEV, PreScission) for highly pure, tag-free final protein products.
Quality Assessment
Comprehensive QC including SDS-PAGE, Western Blot, SEC-HPLC for aggregation status, endotoxin testing, and functional assays.
Pichia pastoris Systems
Utilizing high-density fermentation and the strong AOX1 promoter for cost-effective, high-yield production of secreted proteins.
Automated FPLC Systems
Using advanced Fast Protein Liquid Chromatography (FPLC) systems (e.g., AKTA) for precise, scalable, and reproducible multi-step purification.
Advanced Glycoengineering
Yeast strains engineered to produce humanized glycosylation patterns, essential for therapeutic proteins like monoclonal antibodies.
Therapeutic Proteins and Vaccines
Production of functional protein components for drug development, clinical trials, and vaccine candidates.
Industrial Enzymes
Large-scale production of enzymes for applications in detergents, food processing, textiles, and biofuel production.
Structural Biology Studies
Purification of high-purity protein samples necessary for X-ray crystallography and Cryo-EM analysis.
A guaranteed process from gene to purified protein.
Gene Synthesis & Cloning
Expression Screening
Scale-Up Production
Purification & QC
Gene Design: Codon optimization, addition of purification tags, and cloning into the chosen yeast expression vector.
Transformation: Stable integration of the expression cassette into the yeast host genome.
Pilot Expression: Test expression and solubility in small-scale culture under various induction conditions.
Host Selection: Select the highest-expressing clone for large-scale production.
Fermentation: Scale up the best clone in bioreactors using optimized fed-batch protocols to maximize yield.
Harvest: Collect culture supernatant (for secreted protein) or cell pellet (for intracellular protein).
Chromatography: Multi-step FPLC-based purification scheme tailored to the target protein's properties.
Final QC: Perform final purity analysis, endotoxin removal, and functional testing before shipment.
Functional PTMs and Folding
Superior to E. coli for complex proteins requiring proper eukaryotic folding and post-translational modifications.
High Density and Yield
Ability to reach high cell densities (especially Pichia) in fed-batch fermentation, leading to high protein titers and cost efficiency.
Low Endotoxin Levels
Yeast is inherently endotoxin-free, simplifying purification for proteins intended for in vivo or clinical applications.
Tailored Purification Scheme
Customized multi-step chromatography to achieve >95% purity for even the most challenging target proteins.
"The Pichia pastoris system successfully expressed our complex mammalian cytokine with the correct disulfide bonds and glycosylation, which E. coli could not achieve. The final purity was excellent."
Dr. Helen Zhao, Research Director, Immunology Therapeutics
"The fed-batch fermentation optimization resulted in a dramatic increase in yield—we achieved over 5 grams per liter of our industrial enzyme, making the process commercially viable."
Mr. David Chen, Head of Production, Specialty Enzymes Co.
"The custom FPLC scheme was highly effective. We received a high-purity, tag-free sample of our protein of interest, which was critical for our structural biology studies."
Ms. Anya Sharma, Principal Scientist, Structural Biology Lab
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What is the maximum scale for your yeast fermentation service?
Our standard production scales range up to 50 liters in bioreactors, utilizing optimized fed-batch protocols to maximize cell density and product yield.
Can you perform expression in both Saccharomyces and Pichia?
Yes, we have established, high-efficiency protocols for both Saccharomyces cerevisiae (for certain PTMs and simple proteins) and Pichia pastoris (for high-yield secreted expression).
How do you ensure the purified protein is functional?
In addition to standard purity analysis (SDS-PAGE, SEC), we include customer-specific functional assays (e.g., enzyme kinetics, binding studies) in our quality control upon request to ensure biological activity.
What is the guaranteed purity level?
We typically guarantee a purity level of >90% to >95% as measured by SDS-PAGE and SEC-HPLC, with higher purity achievable based on project specifications and protein characteristics.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.