CD Biosynsis provides expert Yeast Genome Editing Services tailored for metabolic engineering, protein expression, and basic research in yeast host strains, primarily focusing on Saccharomyces cerevisiae and Pichia pastoris. Utilizing cutting-edge technologies, including highly efficient CRISPR/Cas9 Genome Editing , we enable precise and multiplex genetic modifications. Our services cover everything from single Gene Knockout to complex Gene Knock-in for pathway construction, ensuring rapid and accurate modification of the yeast genome. We are committed to accelerating your research and bioproduction goals with validated, high-quality engineered yeast strains.
Get a QuoteYeast, particularly Saccharomyces cerevisiae, is a premier cell factory in industrial biotechnology due to its robust growth, capacity for complex protein modification, and well-characterized genetics. Precise genome editing is fundamental to enhancing its capabilities for producing biofuels, biochemicals, and pharmaceuticals. Our platform is designed to overcome common challenges in yeast transformation and editing, offering superior efficiency and targeting accuracy. We handle projects of all scales, from modifying a single gene to engineering entire metabolic pathways.
Fast, efficient, and multiplexed editing using the CRISPR/Cas9 system for high-volume projects requiring multiple simultaneous modifications.
Precise single-base pair changes (e.g., C>T, A>G) without inducing double-strand breaks, ideal for introducing point mutations or single amino acid substitutions.
CRISPR Multiplexing
Simultaneous targeting of up to five different genomic loci in a single transformation step, significantly accelerating pathway construction.
Stable and complete deletion of target genes, essential for eliminating competing metabolic pathways or assessing gene function.
Precise integration of large DNA fragments, including entire synthetic pathways or heterologous genes, at specific genomic locations.
Tunable and reversible gene knockdown using CRISPR interference (CRISPRi) for fine-tuning metabolic flux or studying essential genes.
Promoter Engineering
Modification of native and synthetic promoter sequences to optimize gene expression levels and regulatory responses to environmental signals.
Chromosomal Integration
Ensuring stable, marker-free integration of transgenes into the yeast chromosome for long-term genetic stability and industrial readiness.
Strain Background Cleanup
Removal of antibiotic resistance markers and unwanted genetic elements to create clean, regulatory-compliant host strains.
Optimized CRISPR/Cas9 Tools
Utilization of yeast-codon-optimized Cas9 variants and efficient guide RNA scaffolds to maximize cleavage efficiency.
Homology-Directed Repair (HDR)
Maximizing the efficiency of the yeast's native HDR pathway for scarless gene insertion and precise point mutation placement.
High-Throughput Verification
Integration of PCR, DNA sequencing, and digital droplet PCR (ddPCR) for rapid and accurate verification of all edits.
Metabolic Pathway Engineering
Creating novel yeast strains capable of producing fuels, chemicals, or natural products by assembling entire heterologous pathways.
Recombinant Protein Production
Optimizing yeast for high yield and correct post-translational modification of therapeutic proteins (e.g., antibodies, growth factors).
Gene Function Studies
Systematic knockout or knockdown libraries for functional genomics screening and identifying novel drug targets.
A structured approach ensuring high success rates and quality.
Design & Synthesis
Transformation & Screening
Verification & Isolation
Final Quality Control
Target Selection: Consultation to finalize target genes and desired modifications (KO, KI, point mutation).
Reagent Synthesis: Design and synthesize guide RNAs (sgRNA) and donor DNA templates.
Cell Preparation: Prepare highly competent yeast cells.
Co-Transformation: Introduce Cas9, sgRNA, and donor DNA (if needed) into the host strain.
Primary Screening: Select for transformants using appropriate markers and plate screening methods.
Edit Verification: Isolate colonies and verify edits using locus-specific PCR and Sanger sequencing.
Marker Removal: If applicable, remove selection markers (scarless editing).
Final QC: Deliver the confirmed, pure engineered yeast strain along with a detailed report.
Guaranteed Knockouts
We offer a guarantee for successful gene knockout for non-essential genes in standard lab yeast strains.
Complex Multiplexing
Expertise in simultaneously editing up to five sites, drastically reducing the time needed for pathway engineering projects.
Scarless Integration
Focus on marker-free and scarless editing techniques that are essential for industrial and regulatory compliance.
Broad Host Strain Compatibility
Services extend beyond S. cerevisiae to include other industrial yeasts like Pichia pastoris and Yarrowia lipolytica.
"The multiplex CRISPR service was highly successful, allowing us to simultaneously knock out three competing pathways and knock in a new synthesis cassette, all in one project timeline."
Dr. Emma Velez, Lead Bioprocess Engineer, Biochemical Production
"We relied on their Base Editing service for targeted point mutations in our enzyme library, resulting in a significantly improved and stabilized protein variant for our manufacturing line."
Mr. Julian Hao, R&D Director, Pharmaceutical Intermediates
"The quality control and sequencing data provided were impeccable, giving us total confidence in the edited strain before proceeding to large-scale fermentation trials."
Ms. Lena Koster, CTO, Nutritional Yeast Products
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What is the typical timeline for a yeast gene knockout project?
A standard single gene knockout project typically takes about 4 to 6 weeks, which includes design, transformation, selection, and comprehensive verification of the final strain.
Can you edit non-conventional yeast species, like Pichia pastoris?
Yes. While S. cerevisiae is our primary host, we have established protocols for editing other industrial yeasts, including Pichia pastoris, Yarrowia lipolytica, and Hansenula polymorpha.
What kind of quality control is performed on the edited strains?
We perform rigorous QC, including locus-specific PCR to confirm integration size, Sanger sequencing of the edited region to verify base accuracy, and often whole-plasmid sequencing to confirm the vector integrity.
Is your CRISPR system marker-free?
We specialize in marker-free and scarless editing strategies using temporary selection cassettes or counter-selection systems, which is crucial for industrial applications.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.