CD Biosynsis provides expert Genetic Circuit Design and Engineering Services to create sophisticated, programmable control systems within living cells. By applying principles from electrical engineering, we design and construct synthetic DNA circuits—including switches, oscillators, and logic gates—that enable precise, tunable, and often inducible control over gene expression and metabolic function. Our services encompass every step from the rational design of novel regulatory elements (Promoters/Enhancers) to the rigorous construction and validation of complex circuits, empowering clients to develop smart cellular therapeutics, advanced biosensors, and highly controlled biomanufacturing platforms.
Get a QuoteGenetic Circuit Engineering is a core discipline of synthetic biology focused on creating novel biological functions by precisely connecting genetic parts (promoters, ribosome binding sites, genes) into integrated regulatory networks. These networks, or circuits, allow cells to sense environmental cues (inputs) and respond predictably (outputs), such as turning on a metabolic pathway only when a substrate is available, or inducing apoptosis only when multiple disease markers are present. The goal is to introduce reliable, programmable logic into biological systems.
Rational design or directed evolution of promoters to achieve specific expression strengths, tunability, or inducibility in a target host.
Creation of novel enhancer sequences to significantly boost basal promoter activity or modify its responsiveness to transcription factors.
High-fidelity assembly of complex, multi-component genetic circuits (Logic Gates, Toggle Switches, Feedback Loops) using advanced DNA techniques.
Rigorous experimental characterization (Flow Cytometry, Time-Lapse Microscopy) to confirm the circuit's predicted function and dynamics.
We follow a systematic Design-Build-Test-Learn cycle to ensure the final circuit is reliable and performs as intended.
Design & Simulation
Part Construction & Assembly
Integration & Screening
Characterization & Refinement
Define the desired circuit function (input/output relationship).
Mathematical modeling and computer simulation to predict circuit dynamics and identify optimal parts.
Selection or design of specific regulatory elements (Promoters, Ribosome binding sites) to meet functional specifications.
High-fidelity synthesis of custom DNA sequences for promoters and functional genes.
Assembly of individual parts into the complete genetic circuit using modular cloning techniques (e.g., Golden Gate).
QC verification of assembled circuits via sequencing.
Detailed analysis of circuit behavior (e.g., measuring response curve, noise, and stability) using advanced analytics.
Compare experimental data against simulation results for debugging and optimization (Learn phase).
Final delivery of the functionally verified cell line and a comprehensive DNA and data package.
Advanced In Silico Modeling
We use robust mathematical and computational tools to predict circuit behavior, significantly increasing the probability of success on the first build.
Proprietary Regulatory Part Libraries
Access to our libraries of characterized Promoters and Enhancers for precise tuning of every component in the circuit.
Functional Circuit Validation
Rigorous testing using Flow Cytometry and other high-resolution methods to guarantee that the circuit performs according to the specifications.
Cross-Host Compatibility
Expertise in designing circuits that function correctly across different hosts, including bacteria, yeast, and mammalian cell lines.
"The team designed a complex AND gate circuit for our therapeutic cell line. The functional validation data was extremely clean and confirmed the predicted logic, allowing us to move quickly to preclinical testing. Their expertise in mammalian circuit design is unmatched."
Dr. Samuel Reyes, Lead Engineer, Cell Therapy Institute
"We relied on their Synthetic Promoter Design service to create an inducible system in E. coli with very low leakage and high induction ratios. This level of control was critical for our toxic product synthesis pathway."
Ms. Clara Dubois, R\&D Manager, Fine Chemicals Corp.
"The Circuit Validation and Testing service provided deep dynamic characterization that revealed hidden instabilities in our design. The subsequent refinement based on their data led to a robust and highly reliable biosensor."
Prof. Akira Sato, University Synthetic Biology Lab
"We commissioned CD Biosynsis to support an intricate gene editing project with multiple targets. Their talent in producing high-quality work in a short period of time was impressive. Their solutions were custom made to suit our needs, and they went above and beyond to ensure our experiments worked. Their support has been a great asset to our research department and we look forward to further working with them."
Dr. Raj Patel, Principal Investigator, Department of Molecular Biology
"As a pharmaceutical company working to discover new cancer therapies, we require accurate, trustworthy gene editing solutions. CD Biosynsis did more than what we expected when it came to providing strong, accurate CRISPR/Cas9 solutions for our preclinical research. Their technical support team was excellent and responsive, and they quickly replied to our questions. This alliance has been pivotal in helping us move our drug pipeline forward. Thank you, CD Biosynsis, for your amazing service!"
Dr. Clara Rodriguez, Chief Scientist, AstraZeneca Pharmaceuticals, Spain
What kind of logic functions can your circuits implement?
We can design circuits to implement standard logic functions such as AND, OR, NOT, NAND, NOR, and more complex multi-input logic required for decision-making in biological systems (e.g., sensing two different disease markers before triggering a therapeutic output).
How do you ensure the robustness and predictability of the final circuit?
Robustness is ensured through: 1) High-quality parts: Using well-characterized promoters/enhancers; 2) Modeling: Simulating circuit behavior under various noise conditions; 3) Rigorous Testing: Characterizing the circuit across the full range of input concentrations and over time (dynamics).
Can you design circuits for mammalian or plant cells?
Yes, our expertise extends across prokaryotic (E. coli, B. subtilis) and eukaryotic systems, including S. cerevisiae, mammalian (CHO, HEK) and plant cells. Circuit design is customized to address the specific transcription and translation machinery of the host.
What is the role of Synthetic Enhancer Design?
Synthetic enhancers are crucial for maximizing the expression level of a circuit's output, especially in eukaryotic systems where gene expression needs to be very high (e.g., for producing large amounts of a therapeutic protein) or when a promoter needs to be highly tunable.
How much does Metabolic Engineering services cost?
The cost of Metabolic Engineering services depends on the project scope, complexity of the target compound, the host organism chosen, and the required yield optimization. We provide customized quotes after a detailed discussion of your specific research objectives.
Do your engineered strains meet regulatory standards?
We adhere to high quality control standards in all strain construction and optimization processes. While we do not handle final regulatory approval, our detailed documentation and compliance with best laboratory practices ensure your engineered strains are prepared for necessary regulatory filings (e.g., GRAS, FDA).
What to look for when selecting the best gene editing service?
We provide various gene editing services such as CRISPR-sgRNA library generation, stable transformation cell line generation, gene knockout cell line generation, and gene point mutation cell line generation. Users are free to select the type of service that suits their research.
Does gene editing allow customisability?
Yes, we offer very customised gene editing solutions such as AAV vector capsid directed evolution, mRNA vector gene delivery, library creation, promoter evolution and screening, etc.
What is the process for keeping data private and confidential?
We adhere to the data privacy policy completely, and all customer data and experimental data are kept confidential.
CRISPR-Cas9 technology represents a transformative advancement in gene editing techniques. The main function of the system is to precisely cut DNA sequences by combining guide RNA (gRNA) with the Cas9 protein. This technology became a mainstream genome editing tool quickly after its 2012 introduction because of its efficient, simple and low-cost nature.
The CRISPR gene editing system with its Cas9 version stands as a vital instrument for current biological research. CRISPR technology enables gene knockout (KO) through permanent gene expression blockage achieved by sequence disruption. Various scientific domains including disease modeling and drug screening employ this technology to study gene functions. CRISPR KO technology demonstrates high efficiency and precision but requires confirmation and verification post-implementation because unsatisfactory editing may produce off-target effects or incomplete gene knockouts which impact experimental result reliability. For precise and efficient Gene Editing Services - CD Biosynsis, Biosynsis offers comprehensive solutions tailored to your research needs.
The CRISPR-Cas9 knockout cell line was developed using CRISPR/Cas9 gene editing to allow scientists to remove genes accurately for research on gene function and disease models and pharmaceutical discovery. Genetic research considers this technology essential due to its high efficiency together with simple operation and broad usability.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.