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TRG Knockout Cell Lines

Gene: TRG

Official Full Name: T cell receptor gamma locusprovided by HGNC

Gene Summary: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable), J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the gamma locus are known to be incapable of encoding a protein and are considered pseudogenes. Somatic rearrangement of the gamma locus has been observed in T cells derived from patients with T cell leukemia and ataxia telangiectasia. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO35067 TRG Knockout cell line (HeLa) Human TRG 1:3~1:6 Negative Online Inquiry
KO35068 TRG Knockout cell line (HCT 116) Human TRG 1:2~1:4 Negative Online Inquiry
KO35069 TRG Knockout cell line (HEK293) Human TRG 1:3~1:6 Negative Online Inquiry
KO35070 TRG Knockout cell line (A549) Human TRG 1:3~1:4 Negative Online Inquiry

Background

TRG Gene Knockout Cell Lines are a sophisticated biological tool designed for precise genetic manipulation in mammalian systems. These cell lines have been engineered to selectively disrupt specific target genes, allowing researchers to investigate gene function, validate drug targets, and model human diseases. Utilizing CRISPR-Cas9 technology, TRG Gene Knockout Cell Lines facilitate efficient and reproducible gene editing, thereby providing a reliable platform for molecular and cellular biology studies.

The key functions of these knockout cell lines involve the introduction of targeted deletions or mutations in the genomic DNA, which can lead to loss-of-function phenotypes that are critical for understanding gene roles. This method enables scientists to dissect complex biological pathways, explore gene interactions, and evaluate therapeutic interventions in a controlled environment. Furthermore, the versatility of these cell lines supports a wide range of applications from basic research to translational studies, significant for the advancement of fields such as cancer biology, neurobiology, and pharmacology.

The scientific importance of TRG Gene Knockout Cell Lines cannot be overstated; they provide a robust model for validating genetic hypotheses and contribute substantially to the development of novel therapeutics. Unlike other gene editing platforms, TRG cell lines ensure a high fidelity in gene targeting with minimal off-target effects, which is critical for achieving reliable experimental outcomes. Additionally, harnessing these knockout lines minimizes the time and resources typically required for generating stable knockout models, thus accelerating the pace of research.

For researchers and clinicians, the ability to manipulate gene expression with such precision offers invaluable insights into disease mechanisms, enabling the identification of potential biomarkers and novel drug targets. Our commitment to quality and innovation has positioned our company as a leader in providing genetically modified cell lines and supporting tools that empower advancements in biomedical research. By choosing TRG Gene Knockout Cell Lines, users not only gain access to cutting-edge technology but also benefit from our extensive expertise and dedicated support in optimizing experiments.

Please note that all services are for research use only. Not intended for any clinical use.

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