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SCUBE3 Knockout Cell Lines

Gene: SCUBE3

Official Full Name: signal peptide, CUB domain and EGF like domain containing 3provided by HGNC

Gene Summary: This gene encodes a member of the signal peptide, complement subcomponents C1r/C1s, Uegf, bone morphogenetic protein-1 and epidermal growth factor-like domain containing protein family. Overexpression of this gene in human embryonic kidney cells results in secretion of a glycosylated form of the protein that forms oligomers and tethers to the cell surface. This gene is upregulated in lung cancer tumor tissue compared to healthy tissue and is associated with loss of the epithelial marker E-cadherin and with increased expression of vimentin, a mesenchymal marker. In addition, the protein encoded by this gene is a transforming growth factor beta receptor ligand, and when secreted by cancer cells, it can be cleaved in vitro to release the N-terminal epidermal growth factor-like repeat domain and the C-terminal complement subcomponents C1r/C1s domain. Both the full length protein and C-terminal fragment can bind to the transforming growth factor beta type II receptor to promote the epithelial-mesenchymal transition and tumor angiogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO00962 SCUBE3 Knockout cell line (Huh-7) Human SCUBE3 1:2~1:3 Negative Online Inquiry
KO00963 SCUBE3 Knockout cell line (Hep G2) Human SCUBE3 Negative Online Inquiry

Background

SCUBE3 Gene Knockout Cell Lines are genetically engineered cellular models specifically designed to investigate the role of the SCUBE3 gene in various biological processes and disease mechanisms. These cell lines exhibit complete disruption of the SCUBE3 gene, providing researchers a powerful tool to study gene function, signaling pathways, and phenotypic consequences associated with its absence. SCUBE3 is known to be involved in important cellular processes such as growth factor signaling and ECM remodeling, making these knockout models invaluable for dissecting its contributions to development and pathology.

The key function of SCUBE3 Gene Knockout Cell Lines lies in their ability to facilitate targeted research in the fields of cancer, cardiovascular diseases, and tissue repair. By eliminating SCUBE3 expression, researchers can examine changes in cellular behavior, including proliferation, differentiation, and apoptosis. Utilizing advanced CRISPR/Cas9 technology, these cell lines allow for precise deletions at the genetic level, ensuring reproducibility and reliability in experimental outcomes.

From a scientific perspective, these knockout cell lines have widespread applications in both basic and translational research settings. They enable elucidation of SCUBE3's role in disease mechanisms, providing insights that can lead to novel therapeutic strategies. The ability to conduct loss-of-function studies without the interference typical of wild-type cellular environments enhances the specificity of experimental results, promoting a deeper understanding of gene function.

The primary advantages that SCUBE3 Gene Knockout Cell Lines offer compared to alternative models include their high specificity and versatility. Unlike traditional knockdown approaches, which may only reduce gene expression, these knockout models achieve complete gene ablation, leading to more definitive insights. Additionally, they are readily adaptable to various experimental designs, which can be particularly advantageous for high-throughput screening or mechanistic studies.

For researchers and clinicians delving into the complexities of gene function and its implications for human health, SCUBE3 Gene Knockout Cell Lines represent a valuable asset. Their ability to provide clear, actionable data in understanding gene regulation and pathway dynamics makes them an essential component of modern biological research. Our company specializes in providing high-quality biological products tailored to advancing scientific discovery, ensuring that researchers have access to innovative tools that can drive their studies forward.

Please note that all services are for research use only. Not intended for any clinical use.

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