Gene: PRKCH
Official Full Name: protein kinase C etaprovided by HGNC
Gene Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO35918 | PRKCH Knockout cell line (HeLa) | Human | PRKCH | 1:3~1:6 | Negative | Online Inquiry |
KO35919 | PRKCH Knockout cell line (HCT 116) | Human | PRKCH | 1:2~1:4 | Negative | Online Inquiry |
KO35920 | PRKCH Knockout cell line (HEK293) | Human | PRKCH | 1:3~1:6 | Negative | Online Inquiry |
KO35921 | PRKCH Knockout cell line (A549) | Human | PRKCH | 1:3~1:4 | Negative | Online Inquiry |
PRKCH Gene Knockout Cell Lines are meticulously engineered cellular models designed to study the functional effects of PRKCH (protein kinase C eta), a crucial member of the protein kinase C family involved in various cellular processes, including cell signaling, growth, and differentiation. By eliminating the PRKCH gene, these knockout cell lines enable researchers to observe the consequent phenotypic changes, elucidating the role of this kinase in physiological and pathological conditions.
The primary mechanism through which the PRKCH Gene Knockout Cell Lines operate is by the targeted inactivation of the PRKCH gene using CRISPR-Cas9 technology. This precise gene-editing tool allows for the removal of the gene sequence responsible for PRKCH expression, ensuring that studies conducted on these lines are reflective of the absence of the target protein. The resulting cell lines provide invaluable insights into the signaling pathways influenced by PRKCH, thereby facilitating research on cancer biology, neurodegenerative diseases, and immune response, among others.
From a scientific perspective, PRKCH Gene Knockout Cell Lines hold significant importance in both basic and translational research. Their application in understanding disease mechanisms, drug discovery, and therapeutic interventions underscores their value in clinical settings, where insights gained can lead to innovative treatment strategies. By comparing the knockout lines with wild-type counterparts, researchers can identify potential therapeutic targets, making them an essential resource in the quest to develop novel treatments.
One of the key advantages of our PRKCH Gene Knockout Cell Lines is their high fidelity and reliability, achieved through rigorous validation processes. Unlike alternative models, which can be confounded by residual protein activity, our knockout lines are verified to ensure complete gene disruption. This robust characteristic provides researchers with confidence in the accuracy of their experimental data and the reproducibility of their findings.
Researchers, clinicians, and pharmaceutical professionals will find immense value in leveraging PRKCH Gene Knockout Cell Lines to advance their work. Their potential to elucidate complex biological processes and identify new therapeutic targets makes them indispensable tools in modern biomedical research.
Our company’s expertise in genetic engineering and biotechnology allows us to deliver high-quality biological products, including these specialized knockout cell lines, tailored to meet the rigorous demands of contemporary research. Through our commitment to innovation and excellence, we empower researchers to push the boundaries of scientific knowledge and improve patient outcomes.
Please note that all services are for research use only. Not intended for any clinical use.
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