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PRIM2 Knockout Cell Lines

Gene: PRIM2

Official Full Name: DNA primase subunit 2provided by HGNC

Gene Summary: This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO35932 PRIM2 Knockout cell line (HeLa) Human PRIM2 1:3~1:6 Negative Online Inquiry
KO35933 PRIM2 Knockout cell line (HCT 116) Human PRIM2 1:2~1:4 Negative Online Inquiry
KO35934 PRIM2 Knockout cell line (HEK293) Human PRIM2 1:3~1:6 Negative Online Inquiry
KO35935 PRIM2 Knockout cell line (A549) Human PRIM2 1:3~1:4 Negative Online Inquiry

Background

PRIM2 Gene Knockout Cell Lines are a cutting-edge biological research tool designed for the study of gene function and the validation of therapeutic targets. Created through the precise application of CRISPR/Cas9 gene-editing technology, these cell lines enable the targeted disruption of the PRIM2 gene, which encodes a critical enzyme involved in DNA replication and repair processes. By effectively silencing this gene, researchers can investigate its role in cellular mechanisms, tumorigenesis, and response to chemotherapeutic agents.

The primary function of PRIM2 Gene Knockout Cell Lines lies in their ability to serve as a reliable model for elucidating the biological consequences of PRIM2 deficiency. This is achieved through the disruption of the gene's coding sequence, leading to a loss of enzyme activity. By assessing phenotypic changes, cellular proliferation rates, and gene expression profiles, scientists can gain insights into the pivotal roles that PRIM2 plays in maintaining genomic stability and cell cycle regulation.

Scientific importance is evident in both research and clinical applications; for instance, these cell lines can facilitate drug discovery by allowing for the identification of potential anti-cancer therapies that exploit PRIM2-related pathways. Furthermore, they serve as invaluable tools for genetic screening, functional genomics, and pathway analysis, thus accelerating the pace of translation from bench to bedside.

Compared to traditional methods of gene knockdown, such as RNA interference, PRIM2 Gene Knockout Cell Lines provide a permanent and specific gene inactivation, eliminating off-target effects associated with transient approaches. This permanence enhances reproducibility in experimental outcomes, a crucial factor for researchers seeking reliable data.

For researchers and clinicians alike, the availability of these knockout cell lines translates to a significant improvement in experimental design, targeting novel therapeutic interventions, and ultimately contributing to advancements in personalized medicine.

Our company prides itself on its commitment to excellence in genetic tools and resources for the scientific community. With our expertise in gene editing and cell line development, we strive to deliver high-quality products that empower researchers to make breakthroughs in understanding complex biological systems.

Please note that all services are for research use only. Not intended for any clinical use.

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