PHKA1 Knockout Cell Lines

Gene: PHKA1

Official Full Name: phosphorylase kinase regulatory subunit alpha 1provided by HGNC

Gene Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO07968	PHKA1 Knockout cell line (HeLa)	Human	PHKA1	1:3~1:6	Negative	Online Inquiry
KO07969	PHKA1 Knockout cell line (HCT 116)	Human	PHKA1	1:2~1:4	Negative	Online Inquiry
KO07970	PHKA1 Knockout cell line (HEK293)	Human	PHKA1	1:3~1:6	Negative	Online Inquiry
KO07971	PHKA1 Knockout cell line (A549)	Human	PHKA1	1:3~1:4	Negative	Online Inquiry

Background

PHKA1 Gene Knockout Cell Lines are genetically engineered cellular models that have been specifically created to disable the PHKA1 gene, which encodes for the regulatory subunit of phosphorylase kinase. This gene plays a crucial role in glycogen metabolism, influencing pathways that govern energy production within cells. By knocking out the PHKA1 gene, researchers can investigate the consequences of its absence on metabolic processes, cellular signaling pathways, and overall cell function.

These cell lines operate by selective gene disruption techniques, such as CRISPR/Cas9 or traditional knockout strategies, which provide an effective means to study gene function and its physiological impact. Researchers utilize PHKA1 knockout cell lines to delve into conditions associated with altered glycogen metabolism, such as glycogen storage diseases and diabetes, thereby enhancing our understanding of these metabolic disorders.

The scientific importance of these models lies in their potential applications in both research and clinical settings. They serve as pivotal tools for drug screening, functional genomics, and investigations into therapeutic interventions aimed at re-establishing normal metabolic function. Furthermore, they offer a clear advantage over other models, such as animal studies, by allowing for more controlled and reproducible experiments while reducing ethical concerns associated with in vivo research.

Researchers and clinicians benefit from the specificity and targeted nature of the PHKA1 Gene Knockout Cell Lines, which facilitate high-throughput analysis and the generation of valuable data that could lead to the development of innovative treatments.

Our company is dedicated to advancing scientific research through the provision of high-quality biological products, and our expertise in genetic engineering has culminated in the development of these PHKA1 Knockout Cell Lines, showcasing our commitment to empowering researchers in their quest to understand and address significant health issues.

Please note that all services are for research use only. Not intended for any clinical use.

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