NLRP3 Knockout Cell Lines

Gene: NLRP3

Official Full Name: NLR family pyrin domain containing 3provided by HGNC

Gene Summary: This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
GP00619	NLRP3 gRNA5-gRNA7 KO plasmid		NLRP3			$850
KO00842	NLRP3 Knockout cell line(THP-1)	Human	NLRP3	1：2-1：4	Negative	Online Inquiry

Background

NLRP3 Gene Knockout Cell Lines are genetically modified cell lines created to specifically disable the NLRP3 gene, which plays a crucial role in the regulation of innate immunity and inflammation. By employing CRISPR-Cas9 technology or other gene-editing techniques, these cell lines provide researchers with a powerful tool to study the function of the NLRP3 inflammasome in various biological processes and disease states, including autoimmune diseases, metabolic disorders, and infectious diseases.

The key mechanism by which NLRP3 functions involves its activation in response to cellular stress signals, leading to the assembly of the inflammasome complex and subsequent release of pro-inflammatory cytokines such as IL-1β and IL-18. Disabling the NLRP3 gene allows for the exploration of this pathway in controlled experimental settings, offering insights into how dysregulation of inflammation contributes to pathophysiological conditions. Researchers can utilize these cell lines to investigate the effects of pharmacological agents, gene therapies, or other interventions designed to modulate the inflammatory response.

The scientific importance of NLRP3 gene knockout cell lines is underscored by their utility in preclinical studies aimed at drug discovery and therapeutic development. These cell lines provide a reliable model for elucidating the mechanisms underlying inflammation, allowing for the validation of biomarkers and therapeutic targets against a backdrop of immune response variability.

What sets our NLRP3 Gene Knockout Cell Lines apart from other models is their high fidelity and reproducibility, ensuring that researchers can obtain consistent, reliable data across experiments. Unlike conventional knockout methods, our engineered cell lines minimize off-target effects and enable easy integration into existing protocols.

By investing in NLRP3 Gene Knockout Cell Lines, researchers and clinicians gain access to a cutting-edge model that facilitates deeper understanding and innovation in immunology and inflammation research. Our company prides itself on its dedication to advancing scientific knowledge through reliable and high-quality biological products, making us a trusted partner in your exploration of complex immunological pathways.

Please note that all services are for research use only. Not intended for any clinical use.

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