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MYLK Knockout Cell Lines

Gene: MYLK

Official Full Name: myosin light chain kinaseprovided by HGNC

Gene Summary: This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO08438 MYLK Knockout cell line (HeLa) Human MYLK 1:3~1:6 Negative Online Inquiry
KO08439 MYLK Knockout cell line (HCT 116) Human MYLK 1:2~1:4 Negative Online Inquiry
KO08440 MYLK Knockout cell line (HEK293) Human MYLK 1:3~1:6 Negative Online Inquiry
KO08441 MYLK Knockout cell line (A549) Human MYLK 1:3~1:4 Negative Online Inquiry

Background

MYLK Gene Knockout Cell Lines are a cutting-edge biotechnological product designed specifically for the study of the myosin light chain kinase (MYLK) gene and its associated pathways. Developed through advanced CRISPR-Cas9 genome editing technology, these cell lines exhibit complete knockout of the MYLK gene, enabling researchers to investigate its function, regulatory mechanisms, and its role in various physiological and pathological contexts.

The primary function of the MYLK Gene Knockout Cell Lines lies in their ability to facilitate the exploration of MYLK-dependent signaling pathways, particularly those involved in smooth muscle contraction, cell migration, and inflammation. By providing a controlled environment where MYLK is absent, these cell lines allow for precise examination of how its loss affects cellular processes, such as cytoskeletal dynamics and signal transduction events.

The scientific importance of these cell lines extends into both research and clinical settings. In a laboratory context, they serve as essential tools for researchers studying cardiovascular diseases, asthma, and cancer, where MYLK's role is paramount. Clinically, understanding the mechanisms behind MYLK dysregulation may lead to novel therapeutic targets and the development of innovative treatment strategies.

Advantages of the MYLK Gene Knockout Cell Lines include their unparalleled specificity and reliability compared to traditional gene editing methods. Unlike transient knockdown strategies that often produce variable results, the permanent knockout ensures consistent cellular behavior across experiments. Furthermore, these lines are fully characterized and validated, simplifying upstream and downstream applications in various protocols.

To researchers and clinicians alike, these cell lines represent a valuable asset in the drive toward elucidating the complexities of MYLK's function and its impact on human health. Their ease of use and robust validation make them a go-to resource for experimental designs aimed at translating basic science into clinically relevant insights.

At our company, we pride ourselves on an unwavering commitment to advancing biological research through innovative products. With expertise in cell line development and genetic editing technologies, we deliver high-quality tools that empower researchers in their quest to unlock the mysteries of cellular function.

Please note that all services are for research use only. Not intended for any clinical use.

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