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MARCHF2 Knockout Cell Lines

Gene: MARCHF2

Official Full Name: membrane associated ring-CH-type finger 2provided by HGNC

Gene Summary: MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM, Mar 2010]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO22782 MARCHF2 Knockout cell line (HeLa) Human MARCHF2 1:3~1:6 Negative Online Inquiry
KO22783 MARCHF2 Knockout cell line (HCT 116) Human MARCHF2 1:2~1:4 Negative Online Inquiry
KO22784 MARCHF2 Knockout cell line (HEK293) Human MARCHF2 1:3~1:6 Negative Online Inquiry
KO22785 MARCHF2 Knockout cell line (A549) Human MARCHF2 1:3~1:4 Negative Online Inquiry

Background

MARCHF2 Gene Knockout Cell Lines are a suite of advanced biological tools designed for investigating the MARCHF2 gene's role in cellular processes. These cell lines have been engineered to precisely disrupt the MARCHF2 gene through targeted gene knockout techniques, utilizing CRISPR-Cas9 technology. By eliminating the expression of this gene, researchers can observe the resultant phenotypic changes, providing critical insights into its function in cellular immunity and protein stability regulation.

The key mechanism underlying these cell lines involves the CRISPR-Cas9 system, which facilitates the precise alteration of the genome. Upon introduction of the CRISPR components, the MARCHF2 gene is effectively rendered non-functional. This results in an observable impact on pathways dependent on MARCHF2, allowing for in-depth studies into its association with various physiological and pathological states, such as immune response mechanisms in autoimmune diseases and its involvement in cancer biology.

The scientific significance of MARCHF2 Gene Knockout Cell Lines cannot be overstated; they provide a powerful platform for both academic and clinical research. In particular, they enable the dissection of complex biological processes and can be utilized in drug discovery efforts, particularly in identifying novel therapeutic targets for diseases related to the immune system and cancer.

In comparison to alternative gene-editing methods such as RNA interference or traditional gene knockout techniques, our MARCHF2 Gene Knockout Cell Lines offer enhanced specificity and efficiency, allowing for a more straightforward validation of gene function without off-target effects. Additionally, these lines are readily available and characterized, minimizing the time required for researchers to generate their own knockout lines.

For researchers and clinicians focused on gene function analysis, understanding disease mechanisms, or developing innovative therapeutic strategies, MARCHF2 Gene Knockout Cell Lines are an invaluable resource. They represent a leap in our ability to explore and manipulate gene function effectively.

As a leader in genetic engineering and cellular biology solutions, our company is committed to providing high-quality products that support cutting-edge research, enabling scientists worldwide to push the boundaries of innovation in a myriad of biological fields.

Please note that all services are for research use only. Not intended for any clinical use.

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