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LAMB2 Knockout Cell Lines

Gene: LAMB2

Official Full Name: laminin subunit beta 2provided by HGNC

Gene Summary: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region. However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron, which does not change the protein sequence, results in a greater abundance of the unspliced form of the transcript than the spliced form. The full-length nature of the spliced transcript is not known. [provided by RefSeq, Aug 2011]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO10254 LAMB2 Knockout cell line (HeLa) Human LAMB2 1:3~1:6 Negative Online Inquiry
KO10255 LAMB2 Knockout cell line (HCT 116) Human LAMB2 1:2~1:4 Negative Online Inquiry
KO10256 LAMB2 Knockout cell line (HEK293) Human LAMB2 1:3~1:6 Negative Online Inquiry
KO10257 LAMB2 Knockout cell line (A549) Human LAMB2 1:3~1:4 Negative Online Inquiry

Background

LAMB2 Gene Knockout Cell Lines are meticulously engineered cellular models that have undergone targeted genetic modification to disrupt the expression of the LAMB2 gene, which encodes the β2 chain of laminin. Laminins are crucial components of the extracellular matrix, playing a vital role in cell adhesion, differentiation, and migration. The LAMB2 gene knockout facilitates the study of the functional pathways associated with laminin and provides a robust platform for investigating its involvement in various physiological and pathological processes, including cancer progression and tissue repair.

The mechanism underlying the functionality of these cell lines lies in their ability to simulate the absence of LAMB2, thus allowing for the dissection of cellular responses that arise from altered extracellular matrix interactions. By devoiding these cells of this key laminin component, researchers can better understand disease mechanisms, identify potential therapeutic targets, and screen for drug responses in a context that closely mimics altered physiological states.

The scientific importance of LAMB2 Gene Knockout Cell Lines is evident in their expansive applications within both basic research and clinical settings. They serve as indispensable tools for studying diseases that involve laminin defects, such as congenital muscular dystrophies and certain types of cancer. Furthermore, these cell lines facilitate advancements in regenerative medicine by providing insights into how altered cellular environments impact tissue engineering strategies.

What sets our LAMB2 Gene Knockout Cell Lines apart from conventional alternatives is their unparalleled specificity and reproducibility. Each line is validated for knockout efficiency and specificity, ensuring reliable results in various experimental setups. Our commitment to high-quality, rigorously tested products means that researchers can depend on the integrity and functionality of our cell lines, allowing them to conduct their studies with confidence.

In an era where precision and efficiency in research are paramount, LAMB2 Gene Knockout Cell Lines represent a critical asset for researchers and clinicians alike. With our extensive experience in developing high-quality biological products, we are dedicated to supporting the scientific community's needs and driving innovation in life sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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