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DBT Knockout Cell Lines

Gene: DBT

Official Full Name: dihydrolipoamide branched chain transacylase E2provided by HGNC

Gene Summary: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO38269 DBT Knockout cell line (HeLa) Human DBT 1:3~1:6 Negative Online Inquiry
KO38270 DBT Knockout cell line (HCT 116) Human DBT 1:2~1:4 Negative Online Inquiry
KO38271 DBT Knockout cell line (HEK293) Human DBT 1:3~1:6 Negative Online Inquiry
KO38272 DBT Knockout cell line (A549) Human DBT 1:3~1:4 Negative Online Inquiry

Background

DBT Gene Knockout Cell Lines are a sophisticated tool designed to facilitate the study of gene function by providing precise models where specific genes have been inactivated through targeted gene editing techniques. These cell lines offer researchers the unique ability to explore the phenotypic consequences of gene disruption, allowing for a deeper understanding of gene roles in cellular processes, disease mechanisms, and therapeutic responses.

The key function of DBT Gene Knockout Cell Lines lies in their mechanism of action, predominately utilizing CRISPR-Cas9 technology for creating targeted mutations. This approach ensures high efficiency and specificity in gene disruption, enabling researchers to develop robust and reproducible models. By knocking out genes of interest, scientists can investigate their pathways and interactions, providing critical insights that are pivotal for advancing biological research, drug discovery, and development of gene therapies.

The scientific importance of these cell lines cannot be overstated. They are indispensable in both basic research to elucidate gene function and in clinical applications, such as identifying therapeutic targets for diseases ranging from cancer to genetic disorders. Moreover, DBT Gene Knockout Cell Lines are engineered to maintain physiologically relevant characteristics, making them suitable for various assays, including drug screening, functional genomics, and biomarker discovery.

In comparison to traditional knockout models or siRNA techniques, DBT Gene Knockout Cell Lines offer distinctive advantages, including enhanced durability, fewer off-target effects, and the ability to generate multiple knockout combinations in a single cell type. This increases the versatility of experimental designs, permitting more straightforward interpretations of gene interactions without compromising biological relevance.

Investing in DBT Gene Knockout Cell Lines is invaluable for researchers and clinicians aiming to push the boundaries of gene research. Their ability to streamline experiments and yield clear, meaningful results makes them a critical component of modern laboratories focused on genomic and translational research.

Our company specializes in providing high-quality biological products, supported by rigorous scientific validation and a commitment to enabling cutting-edge research. We leverage our expertise to ensure that our DBT Gene Knockout Cell Lines meet the highest standards, facilitating breakthroughs in the life sciences.

Please note that all services are for research use only. Not intended for any clinical use.

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