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CST3 Knockout Cell Lines

Gene: CST3

Official Full Name: cystatin Cprovided by HGNC

Gene Summary: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO38381 CST3 Knockout cell line (HeLa) Human CST3 1:3~1:6 Negative Online Inquiry
KO38382 CST3 Knockout cell line (HCT 116) Human CST3 1:2~1:4 Negative Online Inquiry
KO38383 CST3 Knockout cell line (HEK293) Human CST3 1:3~1:6 Negative Online Inquiry
KO38384 CST3 Knockout cell line (A549) Human CST3 1:3~1:4 Negative Online Inquiry

Background

CST3 Gene Knockout Cell Lines are specialized cellular models designed to facilitate the study of the cystatin C (CST3) gene's role in various biological processes, particularly in relation to neurodegenerative diseases and cancer. These cell lines have been genetically modified to disrupt the CST3 gene, enabling researchers to investigate the gene's function, associated pathways, and its implications in health and disease. The knockout mechanism is typically achieved through CRISPR-Cas9 technology, ensuring precise alterations in the DNA sequence, allowing for robust experimental design and reproducibility.

The primary functions of these cell lines include the assessment of cellular responses to external stimuli, the examination of protease inhibition mechanisms mediated by CST3, and the exploration of its contribution to extracellular matrix remodeling. By knocking out CST3, researchers can dissect its involvement in processes such as apoptosis, inflammation, and cellular migration, which are critical in understanding pathophysiological conditions.

In a research context, CST3 Gene Knockout Cell Lines are invaluable for elucidating the molecular pathways linked to Alzheimer’s disease, multiple sclerosis, and various malignancies. Their application extends to drug discovery and testing, where they serve as a platform for evaluating the efficacy of novel therapeutics targeting CST3-related pathways. In clinical settings, these models offer insights into patient-specific responses, aiding personalized medicine approaches.

One of the standout advantages of CST3 Gene Knockout Cell Lines is their specificity and precision, providing a clearer picture than traditional models that may retain some expression of the target gene. Compared to commercially available alternatives, these cell lines come with comprehensive validation data, ensuring reliability and reproducibility in research outcomes.

Researchers and clinicians will find CST3 Gene Knockout Cell Lines particularly valuable due to the increasing recognition of the CST3 protein as a potential therapeutic target. Understanding its regulatory mechanisms could lead to breakthroughs in treatments for debilitating conditions.

Our company brings years of expertise in genomic editing and cellular model development to provide high-quality CST3 Gene Knockout Cell Lines, underscoring our commitment to advancing biomedical research and clinical applications with scientifically rigorous products.

Please note that all services are for research use only. Not intended for any clinical use.

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