AZIN2 Knockout Cell Lines

Gene: AZIN2

Official Full Name: antizyme inhibitor 2provided by HGNC

Gene Summary: The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of antizyme inhibitor 2 has also been observed in brains of patients with Alzheimer's disease. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO30838	AZIN2 Knockout cell line (HCT 116)	Human	AZIN2	1:2~1:4	Negative	Online Inquiry
KO30839	AZIN2 Knockout cell line (A549)	Human	AZIN2	1:3~1:4	Negative	Online Inquiry

Background

AZIN2 Gene Knockout Cell Lines are genetically engineered cell lines specifically designed to lack the expression of the Azin2 gene, a key regulator involved in polyamine metabolism and cellular growth. These cell lines serve as crucial tools for researchers seeking to elucidate the role of AZIN2 in various biological processes, including cellular proliferation, differentiation, and response to stress. By employing CRISPR/Cas9 technology for precise gene editing, AZIN2 Gene Knockout Cell Lines ensure the complete knockout of gene function, providing a reliable platform for in-depth biological investigations.

The key function of these cell lines is to facilitate the study of AZIN2's impact on cellular behaviors and to dissect the underlying molecular mechanisms. Specifically, these knockout models enable researchers to investigate the pathways through which AZIN2 influences cell cycle regulation, apoptosis, and metabolic reprogramming. Moreover, they can be invaluable in pharmacological studies targeting polyamine metabolism, offering insights into potential therapeutic approaches for various diseases, including cancer and neurodegenerative disorders.

The scientific importance of AZIN2 Gene Knockout Cell Lines extends across both basic and translational research. As an essential model in the investigation of gene function, these cell lines can aid in the development of novel biomarkers and therapeutic targets. Researchers benefit from using these knockout models to identify pathways of dysregulated gene activity related to disease states and to test potential interventions.

What sets our AZIN2 Gene Knockout Cell Lines apart from alternative products in the market is the high efficiency and reliability of the knockout process, which is validated by rigorous quality control measures. This means that users can trust that the cell lines exhibit consistent phenotypic traits linked to the absence of the AZIN2 gene, thus ensuring reproducible research outcomes. Additionally, our products are backed by extensive technical support and applications expertise tailored to maximize user satisfaction.

Investing in AZIN2 Gene Knockout Cell Lines not only accelerates research timelines but also enhances the accuracy of experimental data, proving to be a valuable asset for both academic research laboratories and clinical settings. Our company prides itself on a strong foundation in genetic engineering technologies, offering a wide range of premium biological reagents and cell lines that empower researchers in their pursuit of groundbreaking discoveries.

Please note that all services are for research use only. Not intended for any clinical use.

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