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APC2 Knockout Cell Lines

Gene: APC2

Official Full Name: APC regulator of WNT signaling pathway 2provided by HGNC

Gene Summary: This gene encodes a strongly conserved protein that has an N-terminal coiled-coil domain followed by an armadillo domain, five 20-amino acid repeats, and two SAMP domains. This protein promotes the assembly of a multiprotein complex that recruits and phosphorylates the Wnt effector beta-catenin and targets beta-catenin for ubiquitylation and proteasomal degradation. This protein therefore plays a role in the reduction of cytoplasmic levels of beta-catenin which in turn reduces activation of Wnt target genes that play a pivotal role in the pathogenesis of various human cancers. The protein encoded by this gene is closely related to the adenomatous polyposis coli (APC) tumor-suppressor protein and has similar tumor-suppressor effects. This gene also plays a role in actin assembly, cell-cell adhesion, and microtubule network formation through its interaction with cytoskeletal proteins. This gene has its highest expression in the central nervous system and is involved in brain development through cytoskeletal regulation in neurons. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO01350 APC2 Knockout cell line (HeLa) Human APC2 1:3~1:6 Negative Online Inquiry
KO11835 APC2 Knockout cell line (HCT 116) Human APC2 1:2~1:4 Negative Online Inquiry

Background

APC2 Gene Knockout Cell Lines are advanced biological models designed to facilitate the study of the APC2 gene's role in cellular processes. These genetically modified cell lines have undergone precise CRISPR/Cas9-mediated gene editing, resulting in the complete knockout of the APC2 gene, allowing researchers to investigate its function in pathways related to cell proliferation, differentiation, and apoptosis without the interference of the wild-type gene.

The primary function of the APC2 gene is associated with the regulation of p53, a crucial tumor suppressor. By utilizing these knockout cell lines, scientists can elucidate the downstream effects of APC2 absence, examining cellular responses under various stress conditions and understanding its involvement in cancer biology and therapeutic resistance. The mechanisms at play include alterations in cell cycle regulation and imbalances in signaling pathways that could lead to tumorigenesis.

The scientific significance of APC2 Gene Knockout Cell Lines is profound in both research and clinical settings. These models serve as vital tools for drug discovery, allowing researchers to screen potential pharmaceuticals targeting pathways influenced by APC2. They also provide insights into therapeutic strategies that can effectively modulate p53 activity in cancers characterized by APC2 dysregulation.

Compared to other gene knockout models, APC2 Gene Knockout Cell Lines offer unique advantages such as high specificity, minimal off-target effects, and robust scalability. They are developed under stringent quality controls to ensure consistency and reproducibility, making them a more reliable choice for experimental purposes.

For researchers and clinicians, these cell lines represent an invaluable resource in understanding the complexities of cancer biology and potential therapeutic avenues. The detailed insights gained through their use can lead to groundbreaking discoveries and innovations in cancer treatment.

Our company specializes in the development of high-quality biological products, leveraging cutting-edge gene-editing technologies and a commitment to supporting scientific research. By offering APC2 Gene Knockout Cell Lines, we empower the scientific community to delve deeper into the mechanisms of oncogenesis and therapeutic resistance, ultimately contributing to improved healthcare outcomes.

Please note that all services are for research use only. Not intended for any clinical use.

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