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ACLY Knockout Cell Lines

Gene: ACLY

Official Full Name: ATP citrate lyaseprovided by HGNC

Gene Summary: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
GP00153 ACLY gRNA3-gRNA4 KO plasmid ACLY $850
GP01080 ACLY gRNA7-gRNA8 KO plasmid ACLY $850
GP01081 ACLY gRNA5-gRNA6 KO plasmid ACLY $850
KO00350 ACLY Knockout cell line (PANC-1) Human ACLY 1:2-1:4 Negative Online Inquiry
KO00919 ACLY Knockout cell line(A549) Human ACLY 1:3~1:4 Negative Online Inquiry
KO20053 ACLY Knockout cell line (HeLa) Human ACLY 1:3~1:6 Negative Online Inquiry
KO20054 ACLY Knockout cell line (HCT 116) Human ACLY 1:2~1:4 Negative Online Inquiry
KO20055 ACLY Knockout cell line (HEK293) Human ACLY 1:3~1:6 Negative Online Inquiry

Background

ACLY Gene Knockout Cell Lines are genetically modified cell lines designed to specifically disrupt the acetyl-CoA lyase (ACLY) gene. This product serves as a vital tool for researchers investigating metabolic pathways, particularly those involving lipid metabolism and energy homeostasis. By creating a loss-of-function model for the ACLY gene, these cell lines facilitate the detailed study of the consequences of ACLY deficiency within various cellular contexts.

The primary function of ACLY involves the conversion of citrate to acetyl-CoA, a crucial intermediate in the biosynthesis of fatty acids and cholesterol. The knockout of this gene leads to significant alterations in metabolic processes, enabling scientists to explore how ACLY disruption impacts cellular survival, proliferation, and response to environmental stresses. Researchers can leverage these cell lines to elucidate the role of ACLY in diseases such as obesity, diabetes, and cancer, where altered metabolism plays a critical role.

The scientific importance of these cell lines extends to both fundamental research applications and potential clinical implications. In laboratory settings, they provide invaluable insights into the biochemical pathways influenced by ACLY, offering a platform for drug discovery and the development of therapeutic strategies. Furthermore, ACLY gene knockout models can help evaluate the efficacy of novel compounds targeting metabolic disorders.

What sets our ACLY Gene Knockout Cell Lines apart from alternatives is their rigorous validation process, ensuring the precision and reliability of genetic modifications. Each cell line is thoroughly characterized for consistent performance in various assays, providing researchers with robust data for their studies. Additionally, our product's user-friendly protocols and comprehensive support facilitate seamless integration into existing research frameworks.

For researchers and clinicians focused on cutting-edge metabolic research, the ACLY Gene Knockout Cell Lines present an indispensable resource. They empower scientists to further investigate the links between metabolism and disease, unlocking new avenues for therapeutic intervention.

With a firm commitment to scientific excellence and innovation, our company stands at the forefront of providing high-quality biological tools that drive discovery and enhancement in life sciences research. Our expertise in genetic engineering ensures that we deliver products that not only meet but exceed the expectations of our customers, helping them achieve their research goals efficiently and effectively.

Please note that all services are for research use only. Not intended for any clinical use.

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