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ACE Knockout Cell Lines

Gene: ACE

Official Full Name: angiotensin I converting enzymeprovided by HGNC

Gene Summary: This gene encodes an enzyme involved in blood pressure regulation and electrolyte balance. It catalyzes the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This angiotensin converting enzyme (ACE) also inactivates the vasodilator protein, bradykinin. Accordingly, the encoded enzyme increases blood pressure and is a drug target of ACE inhibitors, which are often prescribed to reduce blood pressure. This enzyme additionally plays a role in fertility through its ability to cleave and release GPI-anchored membrane proteins in spermatozoa. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme. This polymorphism, as well as mutations in this gene, have been implicated in a wide variety of diseases including cardiovascular pathophysiologies, psoriasis, renal disease, stroke, and Alzheimer's disease. Regulation of the homologous ACE2 gene may be involved in progression of disease caused by several human coronaviruses, including SARS-CoV and SARS-CoV-2. Alternative splicing results in multiple transcript variants encoding both somatic (sACE) and male-specific testicular (tACE) isoforms. [provided by RefSeq, Sep 2020]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO38274 ACE Knockout cell line (HCT 116) Human ACE 1:2~1:4 Negative Online Inquiry
KO38275 ACE Knockout cell line (HEK293) Human ACE 1:3~1:6 Negative Online Inquiry

Background

ACE Gene Knockout Cell Lines are highly specialized and validated biological tools developed to facilitate the study of gene function and exploration of genetic pathways in mammalian cells. These cell lines are engineered to have specific genes of interest rendered non-functional through the use of advanced genome editing technologies such as CRISPR-Cas9. By creating targeted deletions or mutations in these genes, researchers can evaluate the resulting phenotypic changes, thus gaining insights into the roles and interactions of these genes in cellular processes.

The primary function of ACE Gene Knockout Cell Lines lies in their ability to mimic disease states, which is crucial for both basic research and drug discovery applications. They enable scientists to dissect the molecular mechanisms underlying various biological phenomena, such as cancer development, metabolic disorders, and regenerative medicine. The mechanisms employed involve precise gene targeting that allows for the complete knockout of the gene, leading to a clearer understanding of gene function without the interference of compensatory pathways.

Scientifically, these knockout models represent a transformative tool in research laboratories and clinical settings, allowing for the validation of genetic contributions to diseases. They are essential in the development of novel therapeutics that target specific pathways implicated in pathologies, and can significantly enhance the predictive value of preclinical studies.

Compared to traditional methods of gene silencing, such as RNA interference, which are often transient and less specific, ACE Gene Knockout Cell Lines offer durable and stable genetic modifications. This allows for long-term studies and reproducibility, ultimately leading to more robust data for researchers. Furthermore, the ease of custom modifications provides a unique advantage, enabling researchers to generate tailored cell lines that reflect specific genetic conditions.

The value of ACE Gene Knockout Cell Lines lies in their capacity to foster groundbreaking discoveries that can transform our understanding of gene function and disease mechanisms. For researchers looking to advance their work, these tools offer unparalleled support in unlocking the complexities of genetic regulation and its implications for health.

Our company is dedicated to providing innovative biotechnological solutions that enhance research capabilities and accelerate discoveries in the biological sciences. With state-of-the-art facilities and a team of experienced scientists, we strive to empower researchers and clinicians with the highest quality products to drive scientific advancements.

Please note that all services are for research use only. Not intended for any clinical use.

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