SLC8A1 Knockout Cell Lines

Gene: SLC8A1

Official Full Name: solute carrier family 8 member A1provided by HGNC

Gene Summary: In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM, Apr 2004]

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Products Background

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO08442	SLC8A1 Knockout cell line (HEK293)	Human	SLC8A1	1:3~1:6	Negative	Online Inquiry

Background

SLC8A1 Gene Knockout Cell Lines are specifically engineered cellular models in which the SLC8A1 gene, encoding the sodium-calcium exchanger NCX1, has been inactivated or deleted. This gene plays a crucial role in maintaining intracellular calcium levels, which are vital for various physiological processes, including neuronal excitation and muscle contraction. By employing advanced CRISPR-Cas9 gene-editing technology, these knockout cell lines allow researchers to investigate the nuanced roles of NCX1 in cellular signaling pathways and disease mechanisms.

The SLC8A1 knockout cell lines function through the absence of the NCX1 protein, resulting in altered calcium homeostasis and consequently impacting cellular excitability and contraction. Researchers can utilize these models to elucidate the molecular underpinnings of cardiovascular diseases, neurodegenerative disorders, and muscle pathologies, as the dysfunctional regulation of calcium ions is often implicated in these conditions. Furthermore, these cell lines serve as powerful tools in drug discovery and testing, thereby facilitating the evaluation of therapeutic compounds targeting calcium signaling pathways.

The scientific importance of SLC8A1 Gene Knockout Cell Lines lies in their ability to provide insight into the physiological roles of NCX1, enabling the exploration of fundamental research questions and potential clinical applications. Compared to alternative methods for studying NCX1, such as transient transfection or RNA interference, knockout cell lines offer a stable and permanent alteration that eliminates variability and enhances reproducibility.

For researchers and clinicians, the use of SLC8A1 knockout cell lines is valuable as they provide a clearer understanding of NCX1’s role in both normal and pathophysiological states. This can evolve into significant breakthroughs in developing targeted therapies and improving clinical outcomes in diseases associated with calcium dysregulation.

Our company specializes in delivering high-quality genetic tools and biological products, affirming our commitment to advancing research capabilities. With a focus on precision and reliability, our SLC8A1 Gene Knockout Cell Lines enable professionals in both academic and clinical laboratories to push the boundaries of discovery and innovation.

Please note that all services are for research use only. Not intended for any clinical use.

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