Gene: SLC25A27
Official Full Name: solute carrier family 25 member 27provided by HGNC
Gene Summary: Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO33206 | SLC25A27 Knockout cell line (HeLa) | Human | SLC25A27 | 1:3~1:6 | Negative | Online Inquiry |
KO33207 | SLC25A27 Knockout cell line (HCT 116) | Human | SLC25A27 | 1:2~1:4 | Negative | Online Inquiry |
KO33208 | SLC25A27 Knockout cell line (HEK293) | Human | SLC25A27 | 1:3~1:6 | Negative | Online Inquiry |
SLC25A27 Gene Knockout Cell Lines are genetically engineered cell lines designed to specifically eliminate the function of the SLC25A27 gene, which encodes for a mitochondrial transporter involved in metabolite translocation across the inner mitochondrial membrane. These knockout cell lines serve as powerful tools for studying the physiological and pathological roles of SLC25A27 in cellular metabolism, energy production, and mitochondrial function.
The key function of these cell lines is to provide researchers with an in vitro model to explore the effects of SLC25A27 deficiency. By disrupting this gene, researchers can observe alterations in metabolic pathways, mitochondrial dynamics, and cellular responses to various stressors, offering insights into diseases linked to altered mitochondrial function, such as metabolic disorders and neurodegenerative diseases. The knockout mechanism is accomplished through the application of CRISPR-Cas9 technology, enabling precise editing of the genome with high efficiency, thus facilitating studies involving gene function and regulation.
The scientific importance of SLC25A27 Gene Knockout Cell Lines lies in their applicability across a range of research areas. They are invaluable in drug discovery, where understanding the metabolic impact of potential therapeutic compounds becomes crucial. Clinically, these models can assist in elucidating disease mechanisms and identifying novel biomarkers for therapeutic targets.
Compared to traditional cell lines, these knockout models provide distinct advantages such as increased specificity for targeted investigations and the ability to replicate human pathophysiological conditions more accurately. Additionally, the depletion of SLC25A27 can lead to reproducible phenotypic variations, allowing researchers to explore diverse biological questions.
This product is essential for researchers and clinicians focusing on mitochondrial biology, metabolic diseases, and pharmacology, enabling them to gain deeper insights and enhance the quality of their work. Our company leverages extensive expertise in gene editing and cellular biology, ensuring that our SLC25A27 Gene Knockout Cell Lines meet the highest standards of quality and reliability. By choosing our products, users gain access to rigorous research capabilities that can drive innovative science forward.
Please note that all services are for research use only. Not intended for any clinical use.
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