Gene: SACS
Official Full Name: sacsin molecular chaperoneprovided by HGNC
Gene Summary: This gene encodes the sacsin protein, which includes a UbL domain at the N-terminus, a DnaJ domain, and a HEPN domain at the C-terminus. The gene is highly expressed in the central nervous system, also found in skin, skeletal muscles and at low levels in the pancreas. This gene includes a very large exon spanning more than 12.8 kb. Mutations in this gene result in autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disorder characterized by early-onset cerebellar ataxia with spasticity and peripheral neuropathy. The authors of a publication on the effects of siRNA-mediated sacsin knockdown concluded that sacsin protects against mutant ataxin-1 and suggest that "the large multi-domain sacsin protein is able to recruit Hsp70 chaperone action and has the potential to regulate the effects of other ataxia proteins" (Parfitt et al., PubMed: 19208651). A pseudogene associated with this gene is located on chromosome 11. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO28146 | SACS Knockout cell line (HeLa) | Human | SACS | 1:3~1:6 | Negative | Online Inquiry |
KO28147 | SACS Knockout cell line (HCT 116) | Human | SACS | 1:2~1:4 | Negative | Online Inquiry |
KO28148 | SACS Knockout cell line (HEK293) | Human | SACS | 1:3~1:6 | Negative | Online Inquiry |
KO28149 | SACS Knockout cell line (A549) | Human | SACS | 1:3~1:4 | Negative | Online Inquiry |
SACS Gene Knockout Cell Lines are advanced biological tools designed to facilitate in-depth research of the SACS (spastic ataxia of Charlevoix-Saguenay) gene and its associated pathways. These cell lines have been genetically engineered to contain targeted deletions of the SACS gene, allowing researchers to explore the functional roles of this gene and its implications in neurodegenerative diseases, particularly those associated with ataxia.
The core mechanism behind these knockout cell lines involves the use of CRISPR-Cas9 technology, which enables precise editing of the genome. By disabling the SACS gene, researchers can study the resultant phenotypic changes in cellular behavior, revealing critical insights into the gene’s role in cellular processes such as apoptosis, oxidative stress response, and neuronal signaling pathways. This functional characterization is essential for developing therapeutic approaches to combat conditions arising from SACS dysfunction.
The scientific importance of SACS Gene Knockout Cell Lines is evident in their applications across various research domains, including genetics, neurobiology, and pharmacology. They serve as essential models for the study of gene function and the biochemical pathways involved in the pathogenesis of neurodegenerative disorders. In clinical settings, understanding the mechanisms underpinning SACS-related conditions can lead to innovative treatment strategies, tailored therapies, and even novel drug development.
What sets our SACS Gene Knockout Cell Lines apart from other genetic models is their validated performance, ease of use, and compatibility with high-throughput screening technologies. This ensures that researchers can seamlessly integrate these cell lines into existing workflows, making them invaluable for laboratories focused on cutting-edge research.
In conclusion, SACS Gene Knockout Cell Lines represent a significant advancement in genetic research, offering unparalleled insights into the role of the SACS gene. With our company’s extensive expertise in biological product development, we are committed to providing high-quality, reliable tools that empower researchers and clinicians in their quest for scientific discovery and therapeutic innovation.
Please note that all services are for research use only. Not intended for any clinical use.
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