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PUM1 Knockout Cell Lines

Gene: PUM1

Official Full Name: pumilio RNA binding family member 1provided by HGNC

Gene Summary: This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO32904 PUM1 Knockout cell line (HeLa) Human PUM1 1:3~1:6 Negative Online Inquiry
KO32905 PUM1 Knockout cell line (HCT 116) Human PUM1 1:2~1:4 Negative Online Inquiry
KO32906 PUM1 Knockout cell line (HEK293) Human PUM1 1:3~1:6 Negative Online Inquiry
KO32907 PUM1 Knockout cell line (A549) Human PUM1 1:3~1:4 Negative Online Inquiry

Background

PUM1 Gene Knockout Cell Lines are specifically engineered cellular systems that allow for the precise evaluation of the PUM1 gene's biological role by creating effective loss-of-function models. This product employs advanced CRISPR/Cas9 technology to disrupt the PUM1 gene in relevant cell types, leading to the generation of knockout cell lines that can be utilized in diverse research applications. By elucidating the functional consequences of PUM1 deletion, these cell lines serve as powerful tools to explore gene regulation, cellular response mechanisms, and intricate pathways involved in various diseases.

The key function of PUM1 Gene Knockout Cell Lines lies in their ability to provide insights into the regulatory roles of the PUM1 gene which is crucial for processes such as RNA metabolism, gene expression modulation, and cellular differentiation. When phosphorulated, the PUM1 protein can bind to specific RNA targets, influencing their stability and translation. By utilizing knockout models, researchers can effectively investigate the downstream effects of PUM1 disruption on cellular phenotypes and signaling pathways.

The scientific importance of these cell lines extends to numerous research and clinical applications, particularly in cancer biology, neurobiology, and developmental studies. They enable researchers to model disease conditions, evaluate therapeutic targets, and identify novel pathways linked to PUM1 dysfunction, thereby accelerating the discovery of new interventions in clinical settings.

Compared to other available gene manipulation techniques, the PUM1 Gene Knockout Cell Lines offer exceptional specificity and reliability, as CRISPR/Cas9 enables targeted gene editing with minimal off-target effects. This precision makes these cell lines invaluable for researchers seeking to delineate gene function within complex biological systems. Furthermore, the user-friendly nature of these products allows for rapid implementation in laboratories with varying expertise levels.

For researchers and clinicians, the value of the PUM1 Gene Knockout Cell Lines cannot be overstated; they offer critical insights that may lead to breakthroughs in understanding diseases linked to PUM1 dysregulation. The ability to dissect the function of this important gene paves the way for novel therapeutic strategies.

Our company, with its extensive background in genetic engineering and cell line development, is dedicated to providing high-quality biological products that empower researchers in their quest for knowledge and innovation. We take pride in our commitment to advancing scientific research through the provision of meticulously validated tools that support the advancement of human health.

Please note that all services are for research use only. Not intended for any clinical use.

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