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CDKN1A Knockout Cell Lines

Gene: CDKN1A

Official Full Name: cyclin dependent kinase inhibitor 1Aprovided by HGNC

Gene Summary: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
KO01399 CDKN1A Knockout cell line (HeLa) Human CDKN1A 1:3~1:6 Negative Online Inquiry
KO11326 CDKN1A Knockout cell line (HCT 116) Human CDKN1A 1:2~1:4 Negative Online Inquiry
KO11327 CDKN1A Knockout cell line (HEK293) Human CDKN1A 1:3~1:6 Negative Online Inquiry
KO11328 CDKN1A Knockout cell line (A549) Human CDKN1A 1:3~1:4 Negative Online Inquiry

Background

CDKN1A Gene Knockout Cell Lines are genetically engineered cell lines specifically designed to disable the expression of the CDKN1A gene, which plays a crucial role in regulating the cell cycle through its encoding of the cyclin-dependent kinase inhibitor p21. This product provides researchers with a powerful tool to study the implications of CDKN1A absence on cellular processes such as proliferation, apoptosis, and DNA repair mechanisms. By effectively eliminating the function of p21, scientists can investigate how the loss of this regulatory protein impacts cell cycle progression and overall cellular health.

The key function of CDKN1A Gene Knockout Cell Lines revolves around their ability to mimic specific pathological conditions observed in various cancers or other diseases characterized by dysregulated cell growth. Upon utilizing these cell lines, researchers can elucidate the molecular pathways and gene networks influenced by p21, further advancing our understanding of tumorigenesis and therapeutic resistance. In clinical settings, insights gained from these studies can lead to the development of novel cancer therapies that target cell cycle regulation.

Compared to traditional methods of gene knockdown, such as RNA interference, the CDKN1A Gene Knockout Cell Lines offer a permanent solution for gene inactivation, providing greater stability and reproducibility in experimental outcomes. Moreover, they enable comprehensive long-term studies, enhancing the reliability of findings in drug response and cellular behavior analyses.

The value of these cell lines extends beyond basic biology; they represent a significant advancement for researchers and clinicians aiming to explore innovative treatment strategies and uncover mechanisms underlying disease processes. By leveraging these knockout models, users can accelerate their research and contribute to pivotal discoveries in the field of cancer biology and beyond.

Our company prides itself on delivering high-quality biological research products backed by rigorous scientific validation and expertise. With a focus on cutting-edge technology and user support, we aim to empower researchers in their quest to advance science and medicine.

Please note that all services are for research use only. Not intended for any clinical use.

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