Gene: APEX2
Official Full Name: apurinic/apyrimidinic endodeoxyribonuclease 2provided by HGNC
Gene Summary: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]
Catalog Number | Product Name | Species | Gene | Passage ratio | Mycoplasma testing | Price |
---|---|---|---|---|---|---|
KO00133 | APEX2 Knockout cell line (A549) | Human | APEX2 | 1:3~1:4 | Negative | Online Inquiry |
KO27669 | APEX2 Knockout cell line (HeLa) | Human | APEX2 | 1:3~1:6 | Negative | Online Inquiry |
KO27670 | APEX2 Knockout cell line (HCT 116) | Human | APEX2 | 1:2~1:4 | Negative | Online Inquiry |
KO27671 | APEX2 Knockout cell line (HEK293) | Human | APEX2 | 1:3~1:6 | Negative | Online Inquiry |
APEX2 Gene Knockout Cell Lines represent a cutting-edge tool in genetic engineering utilized for studying gene function, disease mechanisms, and therapeutic targets. These cell lines are meticulously designed to disable specific genes of interest, thereby allowing researchers to analyze the resultant phenotypic changes and infer the roles of those genes.
The APEX2 technology employs the CRISPR-Cas9 system, a revolutionary genome editing technique that allows for precise alterations at specific loci within the genome. By creating double-strand breaks at targeted gene sites, the cell's repair mechanisms facilitate the incorporation of mutations that effectively knockout target gene expression. This mechanism not only simplifies the process of generating knockout models but also enhances the efficiency and accuracy of gene editing outcomes.
The scientific significance of APEX2 Gene Knockout Cell Lines is profound, as they provide invaluable insights into gene function and regulation, elucidating pathways involved in various diseases, including cancer and genetic disorders. In clinical research, these model systems can be harnessed to test drug responses and gene therapies, assessing potential interventions tailored to specific genetic backgrounds.
Compared to alternative knockout systems, such as traditional homologous recombination methods, APEX2 cell lines offer superior speed, scalability, and versatility, drastically reducing the time from conception to experimentation. Furthermore, these cell lines can be readily adapted for high-throughput screening applications, making them a powerful asset for drug discovery and functional genomic studies.
For researchers and clinicians, the APEX2 Gene Knockout Cell Lines hold exceptional value, facilitating groundbreaking research while minimizing costs and resource requirements. They empower users to generate reproducible results and accelerate the pace of discovery in a wide range of biological fields.
With a commitment to excellence and innovation in biological products, our company provides robust solutions designed to propel research forward, enabling scientists to explore the complexities of gene function with confidence and precision.
Please note that all services are for research use only. Not intended for any clinical use.
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