AOC1 Knockout Cell Lines

Gene: AOC1

Official Full Name: amine oxidase copper containing 1provided by HGNC

Gene Summary: This gene encodes a metal-binding membrane glycoprotein that oxidatively deaminates putrescine, histamine, and related compounds. The encoded protein is inhibited by amiloride, a diuretic that acts by closing epithelial sodium ion channels. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Get A Quote

Products Background Case study

Products

Catalog Number	Product Name	Species	Gene	Passage ratio	Mycoplasma testing	Price
KO00017	AOC1 Knockout cell line （NK-92）	Human	AOC1		Negative	Online Inquiry
KO39155	AOC1 Knockout cell line (HeLa)	Human	AOC1	1:3~1:6	Negative	Online Inquiry

Background

AOC1 Gene Knockout Cell Lines represent a significant advancement in genetic research and cellular studies, specifically targeting the amine oxidase copper-containing 1 (AOC1) gene. These cell lines are genetically modified to eliminate the expression of the AOC1 gene, enabling researchers to more accurately study the biological functions and pathways associated with this enzyme, which is involved in various biological processes, including the metabolism of biogenic amines and the modulation of inflammatory responses.

The key function of AOC1 gene knockout cell lines lies in their ability to facilitate the elucidation of AOC1-associated mechanisms by allowing for controlled experimental conditions that isolate the influence of this gene. By comparing the knockout lines with wild-type cells, researchers can observe differences in cellular behavior, responsiveness to stimuli, and metabolic activities. This includes studying how the absence of AOC1 influences cell proliferation, apoptosis, and signal transduction pathways, thereby providing valuable insights into its role in pathological conditions such as cancer and neurodegenerative disorders.

The scientific relevance of AOC1 gene knockout cell lines extends into various research fields, including pharmacology, toxicology, and personalized medicine, where understanding the genetic basis of disease can lead to improved therapeutic strategies. In clinical settings, these cell lines can be utilized to screen for novel compounds that target AOC1-specific pathways, potentially leading to innovative treatments for conditions associated with its dysfunction.

What sets AOC1 gene knockout cell lines apart from alternative gene interference methods, such as transient knockdown approaches, is their stability and reproducibility. Researchers can conduct long-term studies without the variability associated with transient expression systems. This stability ensures a higher degree of confidence in experimental results, making these cell lines invaluable tools for rigorous scientific inquiry.

For researchers and clinicians seeking to deepen their understanding of AOC1's biological roles and its implications in health and disease, AOC1 gene knockout cell lines offer unparalleled opportunities for discovery and innovation. Our company is dedicated to advancing the field of genetic research, providing high-quality, validated cell lines, and harnessing our extensive expertise in molecular biology to support groundbreaking scientific endeavors.

Case study

AOC1 Gene Knockout Cell Lines for Hepatocellular Carcinoma Research

Research Focus

Investigate the function of amine oxidase copper containing 1 (AOC1) in hepatocellular carcinoma (HCC) cell proliferation, migration, and invasion using AOC1 gene knockout (KO) cell lines, and explore its potential as a therapeutic target by modulating the IL-6/JAK/STAT3 signaling pathway.

Background

1. HCC and Unmet Therapeutic Needs

HCC is a leading cause of cancer-related mortality, with limited treatment options for advanced stages. Understanding the molecular mechanisms driving HCC progression is crucial for developing targeted therapies.

2. AOC1 as a Putative Oncogene

AOC1, a copper-containing amine oxidase, is upregulated in gastric cancer and promotes tumor progression. However, its role in HCC remains unclear. Studies suggest AOC1 may regulate cancer cell behavior through metabolic and signaling pathways.

3. IL-6/JAK/STAT3 Pathway in HCC

The IL-6/JAK/STAT3 pathway is frequently activated in HCC, promoting cell proliferation, epithelial-mesenchymal transition (EMT), and treatment resistance. Identifying upstream regulators of this pathway could reveal new therapeutic strategies.

Solution

1. Generation of AOC1 KO Cell Lines

Method: CRISPR/Cas9 technology was used to generate AOC1 KO in human HCC cell lines (Huh-7 and Hep3B2.1-7). Small interfering RNAs (siRNAs) targeting AOC1 were validated for knockdown efficiency, and stable KO lines were established via lentiviral transduction.

Validation: RT-qPCR and western blotting confirmed >80% reduction in AOC1 mRNA and protein expression in KO cells compared to wild-type (WT) controls.

2. Functional Assays in AOC1 KO Cells

Proliferation Inhibition: CCK-8 and colony formation assays showed AOC1 KO significantly reduced Huh-7 and Hep3B2.1-7 cell viability and colony formation capacity. AOC1 overexpression rescued these effects, confirming specificity.

Migration and Invasion Suppression: Wound-healing and Transwell assays revealed AOC1 KO inhibited cell migration and invasion. EMT markers showed increased E-cadherin and decreased N-cadherin/vimentin expression, indicating reduced EMT potential.

ROS and Cytokine Analysis: AOC1 KO decreased intracellular ROS production and IL-6 secretion in HCC cells, linking AOC1 to oxidative stress and cytokine regulation.

3. Signaling Pathway Mechanisms

IL-6/JAK/STAT3 Pathway: Western blotting showed AOC1 KO suppressed phosphorylation of JAK2 and STAT3 (Tyr705), while IL-6 treatment rescued this suppression. GSEA confirmed enrichment of the IL-6/JAK/STAT3 pathway in AOC1-expressing HCC cells.

Therapeutic Synergy: AOC1 KO sensitized HCC cells to IL-6-neutralizing antibodies, reducing proliferation and invasion further.

Conclusion

1. Mechanistic Insights

AOC1 promotes HCC progression through dual mechanisms:

Oxidative Stress Regulation: AOC1-mediated ROS production enhances IL-6 secretion, activating JAK2/STAT3 signaling.

EMT Induction: AOC1 upregulates mesenchymal markers (N-cadherin, vimentin) and downregulates epithelial markers (E-cadherin), facilitating cell migration and invasion.

2. Translational Significance

Biomarker Potential: AOC1 expression correlates with poor differentiation, advanced TNM stage, and lymph node metastasis in HCC patients, making it a prognostic biomarker.

Therapeutic Target: AOC1 KO inhibits HCC cell growth and sensitizes cells to IL-6 pathway inhibitors, suggesting combination therapies targeting AOC1 and IL-6/STAT3 may improve outcomes.

3. Technical Utility

AOC1 KO cell lines provide a robust model for:

Studying AOC1-dependent signaling in HCC.

Screening small-molecule inhibitors of AOC1 or downstream pathways.

Evaluating the role of AOC1 in drug resistance and tumor microenvironment interactions.

This case study establishes AOC1 as a critical regulator of HCC progression and highlights AOC1 KO cell lines as invaluable tools for preclinical research and therapeutic

AOC1 Gene Knockout Cell Lines

Please note that all services are for research use only. Not intended for any clinical use.

Get a free quote

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.