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ACTN1 Knockout Cell Lines

Gene: ACTN1

Official Full Name: actinin alpha 1provided by HGNC

Gene Summary: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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Products Background

Products

Catalog Number Product Name Species Gene Passage ratio Mycoplasma testing Price
GP00215 ACTN1 gRNA1-gRNA2 KO plasmid ACTN1 $850
KO00632 ACTN1 Knockout cell line(293T) Human ACTN1 1:3~1:6 Negative Online Inquiry
KO05444 ACTN1 Knockout cell line (HeLa) Human ACTN1 1:3~1:6 Negative Online Inquiry
KO05445 ACTN1 Knockout cell line (HCT 116) Human ACTN1 1:2~1:4 Negative Online Inquiry
KO05446 ACTN1 Knockout cell line (HEK293) Human ACTN1 1:3~1:6 Negative Online Inquiry
KO05447 ACTN1 Knockout cell line (A549) Human ACTN1 1:3~1:4 Negative Online Inquiry

Background

ACTN1 Gene Knockout Cell Lines are specialized cellular models engineered to lack the expression of the ACTN1 gene, which encodes alpha-actinin-1, a crucial cytoskeletal protein involved in maintaining cellular structure and integrity. These cell lines allow researchers to dissect the functional role of ACTN1, providing insights into its implications in various physiological and pathological processes, including muscle development, cell adhesion, and the dynamics of intracellular signaling.

By utilizing CRISPR/Cas9 genome editing technology, the ACTN1 Gene Knockout Cell Lines are created with high precision, ensuring a complete elimination of the gene's expression. This targeted knockout enables scientists to explore the downstream effects of ACTN1 deficiency on cellular behaviors, interactions, and signaling pathways, making it a vital tool in the study of muscle disorders and other related conditions where actin cytoskeletal dynamics play a significant role.

The scientific importance of this product lies in its application across multiple research fields, including cell biology, neurology, and genetic disorders. Investigations that employ these knockout models can contribute to the understanding of ACTN1-related diseases, thereby aiding in the identification of potential therapeutic targets. Furthermore, these cell lines are invaluable for drug discovery processes, as researchers can assess the efficacy of novel compounds in ACTN1-deficient cellular contexts.

What sets our ACTN1 Gene Knockout Cell Lines apart from alternative products is the rigorous validation and character characterization they undergo, ensuring reproducibility and reliability. Our models demonstrate consistent genotypic and phenotypic outcomes, enhancing experimental reliability for researchers. For those in both academic and clinical settings, having access to robust and targeted tools such as these knockout cell lines can significantly streamline research efforts and enable more precise experimentation.

In choosing our ACTN1 Gene Knockout Cell Lines, users are backed by our company's extensive expertise in cellular models and genetic engineering. We are committed to delivering high-quality biological products that advance research and improve patient outcomes, making us a trusted partner in the scientific community.

Please note that all services are for research use only. Not intended for any clinical use.

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