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Trusted by Leading Research & Pharma Institutions

Custom Bacillus subtilis Expression Service

Endotoxin-free, high-yield protein production powered by the GRAS-status bacterium with superior secretion capability. From milligram to multi-gram scale.

Endotoxin-Free
GRAS Status
High Yield
Learn More

Trusted by leading research and pharmaceutical institutions

Harvard
Pfizer
MIT
Roche
Stanford
Merck

Why Choose Us

No endotoxin/LPS contamination
Natural secretion capability
Up to 50% of total protein
Scalable from mg to multi-gram

Endotoxin-Free

No LPS contamination

Superior Secretion

Direct export to medium

GRAS Status

FDA recognized safe

Success Rate
96%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Why Choose Our Bacillus subtilis Expression Service?

Leverage the power of Bacillus subtilis - the ideal Gram-positive expression host with natural secretion capability, endotoxin-free production, and proven high yields.

Endotoxin-Free Production

No lipopolysaccharide (LPS) contamination. Unlike E. coli, B. subtilis naturally produces proteins without endotoxins, simplifying downstream purification and reducing costs.

  • No expensive endotoxin removal steps
  • Ideal for therapeutic applications
  • Simplified purification workflow

Superior Secretion

Direct protein secretion into culture medium. Avoids cytoplasmic aggregation and inclusion body formation common in E. coli systems.

  • Oxidative extracellular folding
  • Reduced downstream processing
  • Active protein secretion

GRAS Status

FDA recognized safe for food and pharmaceutical applications.

High Expression

Up to 50% of total cellular protein expression levels.

Multiple Systems

Pgrac, T7-based, secretion, and genome integration.

Scalable

From 5 mL screening to 1000 L industrial production.

Ready to Express Your Protein in B. subtilis?

Get a customized quote for your project. Our team will respond within 24 hours.

Technology Platform

Advanced Expression Technology

Multiple expression systems optimized for high-level protein production.

Pgrac Promoter System

Strong IPTG-inducible promoters with tight regulation. Expression levels up to 50% of total cellular protein with excellent induction ratios.

T7-Based System

Phage T7 RNA polymerase-driven expression for maximum transcriptional activity. Ideal for high-level protein production with SCK22 host strain.

Secretion Expression

Multiple signal peptides (AmyQ, SP-lip, AprE) for efficient protein translocation. Direct secretion into medium simplifies purification.

Genome Integration

Stable chromosomal integration for toxic proteins or long-term production. No antibiotic selection required for stable expression.

Host Strain Options

WB800N
8-fold protease-deficient for secretion
168 Marburg
Standard laboratory strain
1012
Common expression host
Custom strains
Genome-reduced strains available
SCK22 derivatives
T7 expression host
Specifications

Service Specifications

Comprehensive specifications to meet your production requirements.

Parameter Specification
Expression Systems Pgrac (IPTG-inducible), T7-based, Constitutive, Secretion
Host Strains WB800N, 168, 1012, Custom strains
Protein Size Up to 150 kDa
Expression Level Up to 50% of total cellular protein
Purity >95% (multi-step chromatography)
Endotoxin <1 EU/mg (therapeutic grade available)
Purification Tags 6xHis, Strep-tag II, GST, MBP, SUMO
Delivery Format Lyophilized powder or solution
Scale mg to multi-gram (5 mL to 1000 L)
All proteins include SDS-PAGE, MS verification, and Certificate of Analysis
Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

1

Gene Design

Codon optimization and vector construction

2

Transform

Host strain selection and transformation

3

Screen

High-throughput clone selection

4

Express

Optimized fermentation and expression

5

Purify

Multi-step chromatography and QC

Applications

Diverse Applications Across Biotechnology

Our expression services support research and development in multiple fields.

Industrial Enzyme Production

B. subtilis is the preferred host for industrial enzyme production, accounting for over 60% of commercially available enzymes. Our expression system delivers high yields with excellent activity retention.

  • Proteases and amylases for food processing
  • Lipases for biodiesel and detergents
  • Cellulases for textile and pulp industries
  • Carbohydrases for animal feed
60%
of commercial enzymes produced in Bacillus species

Therapeutic Proteins

The GRAS status and endotoxin-free nature of B. subtilis make it ideal for producing therapeutic proteins that require high purity and minimal contamination.

  • Antibody fragments (VHH, scFv)
  • Growth factors and cytokines
  • Enzymes for enzyme replacement therapy
  • Vaccine antigens
<1
EU/mg endotoxin levels achievable

Research Proteins

Fast, reliable protein production for structural biology, biochemical studies, and drug discovery research. Flexible scale from mg to gram quantities.

  • Structural biology samples (X-ray, Cryo-EM)
  • Biochemical assay reagents
  • Drug target proteins
  • Multi-subunit protein complexes
>95%
Purity guaranteed for research applications
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"Switching from E. coli to this B. subtilis platform was a game-changer. Our enzyme showed much higher activity and required minimal downstream processing. Highly recommended!"

S
Senior Scientist
Enzyme Development Company

"The secretion capability saved us weeks of work. Protein came out directly into the medium - we went from culture to purified sample in record time. Professional service!"

R
Research Director
Academic Research Institution

"We've used this service for multiple therapeutic protein projects. Consistent quality and professional support every time. Our go-to provider for B. subtilis expression."

L
Lead Researcher
Pharmaceutical Company
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

85 Citations

Integrative expression vectors with Pgrac promoters for inducer-free overproduction of recombinant proteins in Bacillus subtilis

Dinh Thi Minh Tran, Trang Thi Phuong Phan, Thanh Thi Ngoc Doan, et al. Biotechnology Reports. 2020.

Development of Pgrac promoter-based integrative expression vectors enabling high-level protein production without inducer addition in B. subtilis.

View DOI
42 Citations

A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis

Yang W, Zhang J, Chen F, Li H, et al. Bioresources and Bioprocessing. 2022.

Development of a T7-based expression system in B. subtilis achieving 25-40% total protein expression with excellent scalability.

View DOI
18 Citations

Unlocking Green Biomanufacturing Potential: Superior Heterologous Gene Expression with a T7 Integration Overexpression System in Bacillus subtilis

Yao ZY, Yu MJ, Li QQ, et al. ACS Synthetic Biology. 2024.

Dual-module T7 integration system for high-yield protein production without antibiotics or inducers, achieving 6.86 g/L hyaluronic acid.

View DOI
68 Citations

An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation

Fu G, Yue J, Li D, et al. Proceedings of the National Academy of Sciences. 2022.

MATE expression toolkit enabling precise gene expression regulation with up to 790-fold induction for synthetic biology applications.

View DOI
12 Citations

Metabolic Profile of the Genome-Reduced Bacillus subtilis Strain IIG-Bs-27-39: An Attractive Chassis for Recombinant Protein Production

Knöfler L, Pogrebnya A, Mücksch L, et al. ACS Synthetic Biology. 2024.

Characterization of genome-reduced B. subtilis strain with 26.1% genome deletion showing superior performance for heterologous protein secretion.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our B. subtilis expression service.

B. subtilis offers several key advantages: (1) No endotoxin/LPS - eliminates expensive purification steps; (2) Natural secretion - proteins fold correctly in the oxidative extracellular environment; (3) GRAS status - suitable for food and pharmaceutical applications; (4) No codon bias - minimal optimization required for most genes from diverse organisms.
We guarantee >95% purity for all proteins using multi-step chromatography including affinity, ion exchange, and size exclusion chromatography. Higher purity (>98%) is available upon request for structural biology or therapeutic applications. Every batch includes detailed QC documentation.
We offer four main systems: (1) Pgrac promoter - IPTG-inducible with up to 50% expression; (2) T7-based system - highest transcriptional activity for maximum yield; (3) Secretion system - direct protein export with multiple signal peptides; (4) Genome integration - stable chromosomal expression for toxic proteins.
Yes, we support scales from 5 mL screening cultures to 1000 L industrial fermentation. High-density fermentation protocols ensure consistent yields across all scales, with gram-level production readily achievable. Scale-up characterization studies are available for process validation.
We provide multiple strains: WB800N (8-fold protease-deficient, ideal for secretion); 168 Marburg (standard laboratory strain); 1012 (common expression host); and custom genome-reduced strains for enhanced protein production. Our team can recommend the optimal strain for your specific protein.
Every protein includes comprehensive QC: SDS-PAGE analysis for purity, Mass Spectrometry verification for sequence accuracy, Endotoxin testing for safety, and a Certificate of Analysis. Additional QC (HPLC, activity assays, thermal stability analysis) available on request for specialized applications.

Ready to Start Your Project?

Get a customized quote for your Custom Bacillus subtilis Expression Service project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

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