Synonym: TtAgo
Catalog Number | Product Size | Concentration | Price |
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GE0016 | 50 pmol | 1 μM | Online Inquiry |
Product Information | |
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Description | Thermus thermophilus argonaute (TrAgo) is a prokaryotic programmable DNA-endonuclease from the Gram-negative, thermophilic bacterium Thermus thermophilus. As a DNA-guided endonuclease, it requires a 16-18 nucleotide long 5’-phosphorylated single-stranded DNA oligonucleotide guide to target its activity to a specific corresponding sequence on a substrate. TrAgo is active from 65-85°C. It exihibits mild activity on single-stranded RNA, moderate to high activity on double-stranded DNA, and high activity on single-stranded DNA. |
Applications | Single-tube, isothermal generation of the Cas9-sgRNA constructs. |
Properties | |
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Storage Temperature | -20°C |
Storage Conditions | • 10 mM Tris-HCl • 300 mM NaCl • 1 mM DTT • 0.1 mM EDTA • 50% Glycerol • pH 7.4 at 25°C |
Shipping Conditions | Blue ice. |
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