Primer Design and Synthesis Service

Primers (Oligonucleotides) are the essential, short synthetic DNA molecules that initiate enzymatic reactions such as PCR, sequencing, reverse transcription, and gene assembly. The success of almost every molecular biology and synthetic biology experiment hinges on the quality, purity, and thoughtful design of these starting materials. Faulty primers lead to non-specific amplification, poor efficiency, and inaccurate results, requiring costly re-optimization.

CD Biosynsis offers comprehensive Primer Design and Synthesis Service , combining advanced bioinformatics for high-specificity design with chemical synthesis capabilities for high-purity delivery. We specialize in synthesizing standard oligos, complex modified primers, and quantitative PCR (qPCR) probes, guaranteeing optimal performance in your critical experiments. Our expert team ensures rapid turnaround and provides flexible purification options (Desalted, HPLC, PAGE) to match the stringency of your application, from routine genotyping to highly sensitive gene expression analysis.

Highlights

Our commitment to primer quality ensures reliable results for all your experiments:

• Expert Design: Utilize advanced algorithms to design primers with optimal melting temperatures (T}_m$), minimal self-dimerization, and guaranteed high specificity against complex genomes.
• High Purity Guaranteed: Offer multiple purification grades (e.g., HPLC, PAGE) to minimize truncated sequences and contaminants, essential for sensitive applications like qPCR and cloning.
• Extensive Modification Catalog: Provide a vast array of chemical modifications, including fluorescent dyes (e.g., FAM, HEX, VIC), quenchers, phosphorylation, and proprietary minor base changes.
• Fast and Reliable Delivery: Optimized synthesis protocols and automated logistics ensure some of the shortest turnaround times in the industry for standard oligo orders.

Applications

High-quality primers are the starting point for foundational research and complex synthetic constructs:

Quantitative PCR (qPCR)

           

Synthesis of highly efficient, sequence-verified primers and fluorescent probes (e.g., TaqMan) for accurate gene expression quantification.

DNA Sequencing & Library Prep

Synthesis of sequencing primers, indexed adaptors, and barcoded oligos for Next-Generation Sequencing (NGS) library construction.

Gene Assembly & Cloning

Design and synthesis of high-fidelity overlap extension (OE) PCR primers, or long oligos with added restriction sites and homology arms (e.g., for Gibson Assembly).

Site-Directed Mutagenesis (SDM)

Design and synthesis of high-purity, modified primers for introducing precise point mutations into target genes and expression vectors.

Products & Solutions

A comprehensive offering of primer and probe products tailored to your experimental needs.

Standard DNA Oligonucleotides

Synthesis of short, unmodified primers (up to ~200 bases) at multiple scales (25 nmol to $\mu$mol) with standard desalted purification.

Complex Modified Oligos

Offering over 100 chemical modifications, including amino linkers, biotin, conjugation with functional groups, and locked nucleic acids (LNA).

Fluorescent Probes & qPCR Primers

Synthesis of dye-labeled primers and complex FRET/Quencher probes (e.g., TaqMan, Molecular Beacons) requiring high-stringency HPLC purification.

RNA Oligonucleotides (siRNA, miRNA)

Synthesis of high-quality, sequence-verified RNA oligos, including siRNA for gene silencing and customized RNA aptamers.

High-Purity Purification Options

Choice of Desalted, Cartridge (DMT-on), HPLC, or PAGE purification to ensure the purity levels required for your specific application.

Workflow

Our streamlined process ensures efficient design, synthesis, and delivery of your primers and probes:

• Sequence Submission and Design Consultation: Client submits the target gene or template sequence. For complex applications, our bioinformatics team offers complimentary design consultation to optimize specificity, T}_m$, and secondary structure.
• Chemical Synthesis: The oligonucleotide is synthesized chemically base-by-base on an automated synthesizer, starting at the 3' end, using phosphoramidite chemistry.
• Cleavage, Deprotection, and Purification: The oligo is cleaved from the solid support and deprotected. Purification (e.g., Desalted for standard PCR, HPLC for probes) is performed to remove truncated sequences and synthesis byproducts.
• Quality Control (QC): Every oligo undergoes rigorous QC, typically via Mass Spectrometry (MS) to verify the exact molecular weight and length, and Capillary Electrophoresis (CE) to confirm purity.
• Final Delivery and Documentation: The primer is dried or delivered in a chosen buffer. The final package includes the Certificate of Analysis (COA), MS/CE data, and the final sequence file.

We are dedicated to providing the highest level of product quality and technical support:

• Guaranteed Yield: Guaranteed minimum yield is provided based on the synthesis scale and length, ensuring you have enough material for your project.
• Accurate Quantification: All oligos are quantified using UV spectrophotometry (A}_{260) and delivered with precise molar and mass concentrations.
• Free Technical Support: Access to our molecular biology specialists for troubleshooting complex primer design challenges and experimental setup.
• Modification Expertise: Unrivaled experience in synthesizing oligos with complex modifications for applications like single-molecule imaging and structural biology.